ArticleJournal of molecular biology2006
Mutational analysis of sigma70 region 4 needed for appropriation by the bacteriophage T4 transcription factors AsiA and MotA.
Article in Journal of molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- A single rare σ70 variant establishes a unique gene expression pattern in the E. coli pathobiont LF82.Nucleic acids research · 2024Article
- Development of an NLR-ID Toolkit and Identification of Novel Disease-Resistance Genes in Soybean.Plants (Basel, Switzerland) · 2024Article
- Structural basis of σ appropriation.Nucleic acids research · 2019Article
- A novel RNA polymerase-binding protein that interacts with a sigma-factor docking site.Molecular microbiology · 2017Article
- Transcriptomic and Metabolomics Profiling of Phage-Host Interactions between Phage PaP1 andFrontiers in microbiology · 2017Article
- The Xp10 Bacteriophage Protein P7 Inhibits Transcription by the Major and Major Variant Forms of the Host RNA Polymerase via a Common Mechanism.Journal of molecular biology · 2016Article
- Visualizing the phage T4 activated transcription complex of DNA and E. coli RNA polymerase.Nucleic acids research · 2016Article
- Structural basis for promoter specificity switching of RNA polymerase by a phage factor.Genes & development · 2014Article
- In vivo phosphorylation dynamics of the Bordetella pertussis virulence-controlling response regulator BvgA.Molecular microbiology · 2013Article
- The Bordetella pertussis model of exquisite gene control by the global transcription factor BvgA.Microbiology (Reading, England) · 2012Review
- Bacteriophage T4 MotA activator and the β-flap tip of RNA polymerase target the same set of σ70 carboxyl-terminal residues.The Journal of biological chemistry · 2011Article
- Different requirements for σ Region 4 in BvgA activation of the Bordetella pertussis promoters P(fim3) and P(fhaB).Journal of molecular biology · 2011Article
- Genetic evidence for a novel interaction between transcriptional activator SoxS and region 4 of the σ(70) subunit of RNA polymerase at class II SoxS-dependent promoters in Escherichia coli.Journal of molecular biology · 2011Article
- A mutation within the β subunit of Escherichia coli RNA polymerase impairs transcription from bacteriophage T4 middle promoters.Journal of bacteriology · 2010Article
- Transcriptional control in the prereplicative phase of T4 development.Virology journal · 2010Review
- Novel architectural features of Bordetella pertussis fimbrial subunit promoters and their activation by the global virulence regulator BvgA.Molecular microbiology · 2010Article
- Determinants of affinity and activity of the anti-sigma factor AsiA.Biochemistry · 2010Article
- Direct activator/co-activator interaction is essential for bacteriophage T4 middle gene expression.Molecular microbiology · 2009Article
- 6S RNA binding to Esigma(70) requires a positively charged surface of sigma(70) region 4.2.Molecular microbiology · 2009Article
- Activation of transcription initiation by Spx: formation of transcription complex and identification of a Cis-acting element required for transcriptional activation.Molecular microbiology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Transcriptional activation of bacteriophage T4 middle promoters requires sigma70-containing Escherichia coli RNA polymerase, the T4 activator MotA, and the T4 co-activator AsiA. T4 middle promoters contain the sigma70 -10 DNA element. However, these promoters lack the sigma70 -35 element, having instead a MotA box centered at -30, which is bound by MotA. Previous work has indicated that AsiA and MotA interact with region 4 of sigma70, the C-terminal portion that normally contacts -35 DNA and the beta-flap structure in core. AsiA binding prevents the sigma70/beta-flap and sigma70/-35 DNA interactions, inhibiting transcription from promoters that require a -35 element. To test the importance of residues within sigma70 region 4 for MotA and AsiA function, we investigated how sigma70 region 4 mutants interact with AsiA, MotA, and the beta-flap and function in transcription assays in vitro. We find that alanine substitutions at residues 584-588 (region 4.2) do not impair the interaction of region 4 with the beta-flap or MotA, but they eliminate the interaction with AsiA and prevent AsiA inhibition and MotA/AsiA activation. In contrast, alanine substitutions at 551-552, 554-555 (region 4.1) eliminate the region 4/beta-flap interaction, significantly impair the AsiA/sigma70 interaction, and eliminate AsiA inhibition. However, the 4.1 mutant sigma70 is still fully competent for activation if both MotA and AsiA are present. A previous NMR structure shows AsiA binding to sigma70 region 4, dramatically distorting regions 4.1 and 4.2 and indirectly changing the conformation of the MotA interaction site at the sigma70 C terminus. Our analyses provide biochemical relevance for the sigma70 residues identified in the structure, indicate that the interaction of AsiA with sigma70 region 4.2 is crucial for activation, and support the idea that AsiA binding facilitates an interaction between MotA and the far C terminus of sigma70.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.