ArticleThe EMBO journal2006
Ordered assembly of Sld3, GINS and Cdc45 is distinctly regulated by DDK and CDK for activation of replication origins.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 98 citations in OpenAlex.
- Chimeric nanoparticles for targeting mitochondria in cancer cells.Nanoscale advances · 2022Article
- Article
- GINS2 regulates cell proliferation and apoptosis in human epithelial ovarian cancer.Oncology letters · 2018Article
- The drinking water contaminant dibromoacetonitrile delays G1-S transition and suppresses Chk1 activation at broken replication forks.Scientific reports · 2017Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Review
- Regulation of SLD5 gene expression by miR-370 during acute growth of cancer cells.Scientific reports · 2016Article
- Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation.The EMBO journal · 2016Article
- Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.Nature communications · 2016Article
- A reconstituted system reveals how activating and inhibitory interactions control DDK dependent assembly of the eukaryotic replicative helicase.Nucleic acids research · 2015Article
- Evolutionary conservation of the CDK targets in eukaryotic DNA replication initiation.Chromosoma · 2015Review
- Switch on the engine: how the eukaryotic replicative helicase MCM2-7 becomes activated.Chromosoma · 2015Review
- RecQL4 is required for the association of Mcm10 and Ctf4 with replication origins in human cells.Cell cycle (Georgetown, Tex.) · 2015Article
- MYC and the control of DNA replication.Cold Spring Harbor perspectives in medicine · 2014Review
- Helicase activation and establishment of replication forks at chromosomal origins of replication.Cold Spring Harbor perspectives in biology · 2013Review
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- Coordinated degradation of replisome components ensures genome stability upon replication stress in the absence of the replication fork protection complex.PLoS genetics · 2013Article
- Requirement of SLD5 for early embryogenesis.PloS one · 2013Article
- Functional analysis of Drosophila DNA polymerase ε p58 subunit.American journal of cancer research · 2013Article
- Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast.Genes & development · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Initiation of chromosome DNA replication in eukaryotes is tightly regulated through assembly of replication factors at replication origins. Here, we investigated dependence of the assembly of the initiation complex on particular factors using temperature-sensitive fission yeast mutants. The psf3-1 mutant, a GINS component mutant, arrested with unreplicated DNA at the restrictive temperature and the DNA content gradually increased, suggesting a defect in DNA replication. The mutation impaired GINS complex formation, as shown by pull-down experiments. Chromatin immunoprecipitation assays indicated that GINS integrity was required for origin loading of Psf2, Cut5 and Cdc45, but not Sld3. In contrast, loading of Psf2 onto origins depended on Sld3 and Cut5 but not on Cdc45. These results suggest that Sld3 functions furthest upstream in initiation complex assembly, followed by GINS and Cut5, then Cdc45. Consistent with this conclusion, Cdc7-Dbf4 kinase (DDK) but not cyclin-dependent kinase (CDK) was required for Sld3 loading, whereas recruitment of the other factors depended on both kinases. These results suggest that DDK and CDK regulate distinct steps in activation of replication origins in fission yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.