ArticleStem cells (Dayton, Ohio)2007
Production of green fluorescent protein transgenic embryonic stem cells using the GENSAT bacterial artificial chromosome library.
Article in Stem cells (Dayton, Ohio), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Hippocampal versus cortical deletion of cholinergic receptor muscarinic 1 in mice differentially affects post-translational modifications and supramolecular assembly of respiratory chain-associated proteins, mitochondrial ultrastructure, and respiration: implications in Alzheimer's disease.Frontiers in cell and developmental biology · 2023Article
- LiPS-A3S, a human genomic site for robust expression of inserted transgenes.Molecular therapy. Nucleic acids · 2016Article
- Duration of culture and sonic hedgehog signaling differentially specify PV versus SST cortical interneuron fates from embryonic stem cells.Development (Cambridge, England) · 2015Article
- Identification of transcriptional regulatory elements for Ntng1 and Ntng2 genes in mice.Molecular brain · 2014Article
- Enhanced derivation of mouse ESC-derived cortical interneurons by expression of Nkx2.1.Stem cell research · 2013Article
- Identification of embryonic stem cell-derived midbrain dopaminergic neurons for engraftment.The Journal of clinical investigation · 2012Article
- A novel reporter of notch signalling indicates regulated and random Notch activation during vertebrate neurogenesis.BMC biology · 2011Article
- Derivation of genetically modified human pluripotent stem cells with integrated transgenes at unique mapped genomic sites.Nature protocols · 2011Article
- Identification of a novel intronic enhancer responsible for the transcriptional regulation of musashi1 in neural stem/progenitor cells.Molecular brain · 2011Article
- Pluripotent stem cells for the study of CNS development.Frontiers in molecular neuroscience · 2011Article
- Prospective isolation of cortical interneuron precursors from mouse embryonic stem cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling.Nature biotechnology · 2009Article
- Integration of functional bacterial artificial chromosomes into human cord blood-derived multipotent stem cells.Gene therapy · 2009Article
- Human embryonic stem cells which express hrGFP in the undifferentiated state and during dopaminergic differentiation.Restorative neurology and neuroscience · 2009Article
- Marking embryonic stem cells with a 2A self-cleaving peptide: a NKX2-5 emerald GFP BAC reporter.PloS one · 2008Article
- High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells.Cell stem cell · 2008Article
- Neuregulin1 and ErbB expression in the uninjured and regenerating olfactory mucosa.Gene expression patterns : GEPArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Transgenic green fluorescent protein (GFP) reporter embryonic stem (ES) cells are powerful tools for studying gene regulation and lineage choice during development. Here we present a rapid method for the generation of ES cells expressing GFP under the control of selected genes. Bacterial artificial chromosomes (BACs) from a previously constructed GFP transcriptional fusion library (Gene Expression Nervous System Atlas [GENSAT]) were modified for use in ES cells, and multiple BAC transgenic ES cell lines were generated. Specific GFP expression in transgenic cell lines was confirmed during neural differentiation marking neural stem cells, neuronal precursors, and glial progeny by Hes5, Dll1, and GFAP, respectively. GFP was dynamically regulated in ES cell progeny in response to soluble factors that inhibit Notch signaling and a factor that directs astroglial fate choice. Our protocols provide a simple and efficient strategy to utilize the whole GENSAT BAC library to create hundreds of novel fluorescent cell lines for use in ES cell biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.