ArticleJournal of cellular and molecular medicine
A new approach to tissue engineering of vascularized skeletal muscle.
Article in Journal of cellular and molecular medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Review
- Organoid-based tissue engineering for advanced tissue repair and reconstruction.Materials today. Bio · 2025Review
- Integrating Physical and Biochemical Cues for Muscle Engineering: Scaffolds and Graft Durability.Bioengineering (Basel, Switzerland) · 2024Review
- Effect of niche components on masseter satellite cell differentiation on fibrin coatings.European journal of oral sciences · 2022Article
- Advanced Techniques for Skeletal Muscle Tissue Engineering and Regeneration.Bioengineering (Basel, Switzerland) · 2020Review
- Combining Vascularization Strategies in Tissue Engineering: The Faster Road to Success?Frontiers in bioengineering and biotechnology · 2020Article
- Bringing Safe and Standardized Cell Therapies to Industrialized Processing for Burns and Wounds.Frontiers in bioengineering and biotechnology · 2020Article
- Vascularized and Innervated Skeletal Muscle Tissue Engineering.Advanced healthcare materials · 2020Review
- Holistic Approach of Swiss Fetal Progenitor Cell Banking: Optimizing Safe and Sustainable Substrates for Regenerative Medicine and Biotechnology.Frontiers in bioengineering and biotechnology · 2020Article
- Article
- Convergence of Scaffold-Guided Bone Reconstruction and Surgical Vascularization Strategies-A Quest forFrontiers in bioengineering and biotechnology · 2019Article
- Ectopic BAT mUCP-1 overexpression in SKM by delivering a BMP7/PRDM16/PGC-1a gene cocktail or single PRMD16 using non-viral UTMD gene therapy.Gene therapy · 2018Article
- iPSCs-based generation of vascular cells: reprogramming approaches and applications.Cellular and molecular life sciences : CMLS · 2018Review
- Genetically engineered human muscle transplant enhances murine host neovascularization and myogenesis.Communications biology · 2018Article
- Elderly Patient-Derived Endothelial Cells for Vascularization of Engineered Muscle.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- A short discourse on vascular tissue engineering.NPJ Regenerative medicine · 2017Article
- The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering.Journal of visualized experiments : JoVE · 2016Article
- Striated muscle function, regeneration, and repair.Cellular and molecular life sciences : CMLS · 2016Review
- Bone Graft Prefabrication Following the In Vivo Bioreactor Principle.EBioMedicine · 2016Review
- Ultrasound-guided photoacoustic imaging-directed re-endothelialization of acellular vasculature leads to improved vascular performance.Acta biomaterialia · 2016Article
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Authors and funding
7 authors.
Funding
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Abstract
Tissue Engineering of skeletal muscle tissue still remains a major challenge. Every neo-tissue construct of clinically relevant dimensions is highly dependent on an intrinsic vascularisation overcoming the limitations of diffusion conditioned survival. Approaches incorporating the arteriovenous-loop model might bring further advances to the generation of vascularised skeletal muscle tissue. In this study 12 syngeneic rats received transplantaion of carboxy-fluorescine diacetate-succinimidyl ester (CFDA)-labelled, expanded primary myoblasts into a previously vascularised fibrin matrix, containing a microsurgically created AV loop. As control cells were injected into fibrin-matrices without AV-loops. Intra-arterial ink injection followed by explantation was performed 2, 4 and 8 weeks after cell implantation. Specimens were evaluated for CFDA, MyoD and DAPI staining, as well as for mRNA expression of muscle specific genes. Results showed enhanced fibrin resorption in dependence of AV loop presence. Transplanted myoblasts could be detected in the AV loop group even after 8 weeks by CFDA-fluorescence, still showing positive MyoD staining. RT-PCR revealed gene expression of MEF-2 and desmin after 4 weeks on the AVloop side, whereas expression analysis of myogenin and MHC(embryo) was negative. So far myoblast injection in the microsurgical rat AV loop model enhances survival of the cells, keeping their myogenic phenotype, within pre-vascularised fibrin matrices. Probably due to the lack of potent myogenic stimuli and additionally the rapid resorption of the fibrin matrix, no formation of skeletal muscle-like tissue could be observed. Thus further studies focussing on long term stability of the matrix and the incorporation of neural stimuli will be necessary for generation of vascularised skeletal muscle tissue.
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