ArticleThe Journal of membrane biology2006
Use of steady-state laurdan fluorescence to detect changes in liquid ordered phases in human erythrocyte membranes.
Article in The Journal of membrane biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Slow freezing coupled static magnetic field exposure enhances cryopreservative efficiency--a study on human erythrocytes.PloS one · 2013Trial
- Streptozotocin and L-Buthionine-Sulfoximine Decrease Neuron Membrane Lipid Packing and Alter Insulin Signaling.Neurotoxicity research · 2025Article
- Review
- LAURDAN since Weber: The Quest for Visualizing Membrane Heterogeneity.Accounts of chemical research · 2021Article
- Recent progress on lipid lateral heterogeneity in plasma membranes: From rafts to submicrometric domains.Progress in lipid research · 2016Review
- Article
- Lpcat3-dependent production of arachidonoyl phospholipids is a key determinant of triglyceride secretion.eLife · 2015Article
- Laurdan monitors different lipids content in eukaryotic membrane during embryonic neural development.Cell biochemistry and biophysics · 2014Article
- A robust and quantitative method for tracking liposome contents after intravenous administration.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Imaging the early stages of phospholipase C/sphingomyelinase activity on vesicles containing coexisting ordered-disordered and gel-fluid domains.Journal of lipid research · 2011Article
- Article
- Sequence of physical changes to the cell membrane during glucocorticoid-induced apoptosis in S49 lymphoma cells.Biophysical journal · 2009Article
- Relationship between membrane physical properties and secretory phospholipase A2 hydrolysis kinetics in S49 cells during ionophore-induced apoptosis.Biophysical journal · 2007Article
- Differential detection of phospholipid fluidity, order, and spacing by fluorescence spectroscopy of bis-pyrene, prodan, nystatin, and merocyanine 540.Biophysical journal · 2006Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
In artificial phospholipid bilayers, dual measurements of laurdan steady-state anisotropy and emission spectra can be used to identify the presence of liquid ordered phases. Human erythrocytes were used as a model to test whether similar measurements could be applied to biological samples. Specifically, laurdan anisotropy and emission spectra were obtained from native erythrocytes before and after treatment with calcium ionophore and from the microvesicles (known to be enriched in liquid ordered domains) shed from the cells during calcium entry. Spectral and anisotropy data were consistent with an increased order and reduced fluidity of erythrocyte membrane lipids upon ionophore treatment. Microvesicle membranes appeared more ordered than native erythrocytes and similar to ionophore-treated cells based on laurdan emission. In contrast, the anisotropy value was lower in microvesicles compared to ionophore-treated cells, suggesting greater probe mobility. Parallel measurements of diphenylhexatriene anisotropy corroborated the laurdan data. These results were consistent with the liquid ordered property of microvesicle membranes based on comparisons to behavior in artificial membranes. Two-photon microscopy was used to examine the distribution of laurdan fluorescence along the surface of erythrocyte membranes before and after ionophore treatment. A dual spatial analysis of laurdan anisotropy, as revealed by the distribution of laurdan emission spectra, and intensity excited by polarized light suggested that the plasma membranes of ionophore-treated erythrocytes may also exhibit elevated numbers of liquid ordered domains.
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