ArticleMolecular biology of the cell2006
Intranuclear binding kinetics and mobility of single native U1 snRNP particles in living cells.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- Predicting human mRNA isoform levels from site-specific splicing kineticsbioRxiv : the preprint server for biology · 2026Article
- Imaging Organization of RNA Processing within the Nucleus.Cold Spring Harbor perspectives in biology · 2021Review
- An in vitro reconstituted U1 snRNP allows the study of the disordered regions of the particle and the interactions with proteins and ligands.Nucleic acids research · 2021Article
- An intrinsically disordered region-mediated confinement state contributes to the dynamics and function of transcription factors.Molecular cell · 2021Article
- Inferring quantity and qualities of superimposed reaction rates from single molecule survival time distributions.Scientific reports · 2020Article
- Single molecule tracking reveals that the bacterial SMC complex moves slowly relative to the diffusion of the chromosome.Nucleic acids research · 2018Article
- Diatrack particle tracking software: Review of applications and performance evaluation.Traffic (Copenhagen, Denmark) · 2017Review
- Nuclear accessibility of β-actin mRNA is measured by 3D single-molecule real-time tracking.The Journal of cell biology · 2015Article
- The Supraspliceosome - A Multi-Task Machine for Regulated Pre-mRNA Processing in the Cell Nucleus.Computational and structural biotechnology journal · 2014Review
- Single molecule fluorescence approaches shed light on intracellular RNAs.Chemical reviews · 2014Review
- Dissecting non-coding RNA mechanisms in cellulo by Single-molecule High-Resolution Localization and Counting.Methods (San Diego, Calif.) · 2013Article
- A benchmark for chromatin binding measurements in live cells.Nucleic acids research · 2012Article
- Theory on the coupled stochastic dynamics of transcription and splice-site recognition.PLoS computational biology · 2012Article
- A perspective of the dynamic structure of the nucleus explored at the single-molecule level.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Review
- RNA processing and export.Cold Spring Harbor perspectives in biology · 2010Review
- The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells.The Journal of cell biology · 2010Article
- FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know?Current opinion in cell biology · 2010Review
- Nuclear physics: quantitative single-cell approaches to nuclear organization and gene expression.Cold Spring Harbor symposia on quantitative biology · 2010Article
- Autonomy and robustness of translocation through the nuclear pore complex: a single-molecule study.The Journal of cell biology · 2008Article
- Imaging and tracking of single hyaluronan molecules diffusing in solution.Glycoconjugate journal · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uridine-rich small nuclear ribonucleoproteins (U snRNPs) are splicing factors, which are diffusely distributed in the nucleoplasm and also concentrated in nuclear speckles. Fluorescently labeled, native U1 snRNPs were microinjected into the cytoplasm of living HeLa cells. After nuclear import single U1 snRNPs could be visualized and tracked at a spatial precision of 30 nm at a frame rate of 200 Hz employing a custom-built microscope with single-molecule sensitivity. The single-particle tracks revealed that most U1 snRNPs were bound to specific intranuclear sites, many of those presumably representing pre-mRNA splicing sites. The dissociation kinetics from these sites showed a multiexponential decay behavior on time scales ranging from milliseconds to seconds, reflecting the involvement of U1 snRNPs in numerous distinct interactions. The average dwell times for U1 snRNPs bound at sites within the nucleoplasm did not differ significantly from those in speckles, indicating that similar processes occur in both compartments. Mobile U1 snRNPs moved with diffusion constants in the range from 0.5 to 8 microm2/s. These values were consistent with uncomplexed U1 snRNPs diffusing at a viscosity of 5 cPoise and U1 snRNPs moving in a largely restricted manner, and U1 snRNPs contained in large supramolecular assemblies such as spliceosomes or supraspliceosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.