ArticleMolecular biology of the cell2006
Global organization and function of mammalian cytosolic proteasome pools: Implications for PA28 and 19S regulatory complexes.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 79 citations in OpenAlex.
- PI31 is a positive regulator of 20S immunoproteasome assembly.Journal of cell science · 2025Article
- The Significant Role of PA28αβ in CD8International journal of molecular sciences · 2024Article
- Article
- Downregulation of PA28α induces proteasome remodeling and results in resistance to proteasome inhibitors in multiple myeloma.Blood cancer journal · 2020Article
- The ubiquitin-proteasome system in kidney physiology and disease.Nature reviews. Nephrology · 2019Review
- Visualizing Proteasome Activity and Intracellular Localization Using Fluorescent Proteins and Activity-Based Probes.Frontiers in molecular biosciences · 2019Article
- PIP30/FAM192A is a novel regulator of the nuclear proteasome activator PA28γ.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Detection of active proteasome structures in brain extracts: proteasome features of August rat brain with violations in monoamine metabolism.Oncotarget · 2017Article
- Catalytically Active Proteasomes Function Predominantly in the Cytosol.The Journal of biological chemistry · 2016Article
- Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes.The Journal of biological chemistry · 2016Article
- Native structure of rat liver immune proteasomes.Doklady. Biochemistry and biophysics · 2016Article
- Mass Spectrometry: A Technique of Many Faces.Quarterly reviews of biophysics · 2016Review
- Regulated protein turnover: snapshots of the proteasome in action.Nature reviews. Molecular cell biology · 2014Review
- Redox regulation of the proteasome via S-glutathionylation.Redox biology · 2013Review
- Endoplasmic reticulum protein quality control is determined by cooperative interactions between Hsp/c70 protein and the CHIP E3 ligase.The Journal of biological chemistry · 2013Article
- Subcellular distribution and dynamics of active proteasome complexes unraveled by a workflow combining in vivo complex cross-linking and quantitative proteomics.Molecular & cellular proteomics : MCP · 2013Article
- Context-dependent resistance to proteolysis of intrinsically disordered proteins.Protein science : a publication of the Protein Society · 2011Review
- Protein folding and quality control in the endoplasmic reticulum: Recent lessons from yeast and mammalian cell systems.Current opinion in cell biology · 2011Review
- Not4 E3 ligase contributes to proteasome assembly and functional integrity in part through Ecm29.Molecular and cellular biology · 2011Article
- Ubiquitin-proteasome system profiling in acute leukemias and its clinical relevance.Leukemia research · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Proteolytic activity of the 20S proteasome is regulated by activators that govern substrate movement into and out of the catalytic chamber. However, the physiological relationship between activators, and hence the relative role of different proteasome species, remains poorly understood. To address this problem, we characterized the total pool of cytosolic proteasomes in intact and functional form using a single-step method that bypasses the need for antibodies, proteasome modification, or column purification. Two-dimensional Blue Native(BN)/SDS-PAGE and tandem mass spectrometry simultaneously identified six native proteasome populations in untreated cytosol: 20S, singly and doubly PA28-capped, singly 19S-capped, hybrid, and doubly 19S-capped proteasomes. All proteasome species were highly dynamic as evidenced by recruitment and exchange of regulatory caps. In particular, proteasome inhibition with MG132 markedly stimulated PA28 binding to exposed 20S alpha-subunits and generated doubly PA28-capped and hybrid proteasomes. PA28 recruitment virtually eliminated free 20S particles and was blocked by ATP depletion. Moreover, inhibited proteasomes remained stably associated with distinct cohorts of partially degraded fragments derived from cytosolic and ER substrates. These data establish a versatile platform for analyzing substrate-specific proteasome function and indicate that PA28 and 19S activators cooperatively regulate global protein turnover while functioning at different stages of the degradation cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.