ArticleThe Journal of cell biology1990
A Dictyostelium mutant lacking an F-actin cross-linking protein, the 120-kD gelation factor.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 108 citations in OpenAlex.
- Article
- Domain Organization of the UBX Domain Containing Protein 9 and Analysis of Its Interactions With the Homohexameric AAA + ATPase p97 (Valosin-Containing Protein).Frontiers in cell and developmental biology · 2021Article
- Differential Roles of Actin Crosslinking Proteins Filamin and α-Actinin in Shear Flow-Induced Migration ofFrontiers in cell and developmental biology · 2021Article
- Article
- Ate1-mediated posttranslational arginylation affects substrate adhesion and cell migration in Dictyostelium discoideum.Molecular biology of the cell · 2019Article
- Article
- Expression of N471D strumpellin leads to defects in the endolysosomal system.Disease models & mechanisms · 2018Article
- The phenotypes of ATG9, ATG16 and ATG9/16 knock-out mutants imply autophagy-dependent and -independent functions.Open biology · 2015Article
- Identification of the protein kinases Pyk3 and Phg2 as regulators of the STATc-mediated response to hyperosmolarity.PloS one · 2014Article
- Heteromeric p97/p97R155C complexes induce dominant negative changes in wild-type and autophagy 9-deficient Dictyostelium strains.PloS one · 2012Article
- Regulation of the actin cytoskeleton by an interaction of IQGAP related protein GAPA with filamin and cortexillin I.PloS one · 2010Article
- A cytohesin homolog in Dictyostelium amoebae.PloS one · 2010Article
- RNAi silenced Dd-grp94 (Dictyostelium discoideum glucose-regulated protein 94 kDa) cell lines in Dictyostelium exhibit marked reduction in growth rate and delay in development.Gene expression · 2010Article
- Actin binding domains direct actin-binding proteins to different cytoskeletal locations.BMC cell biology · 2008Article
- Filamin repeat segments required for photosensory signalling in Dictyostelium discoideum.BMC cell biology · 2007Article
- Villidin, a novel WD-repeat and villin-related protein from Dictyostelium, is associated with membranes and the cytoskeleton.Molecular biology of the cell · 2003Article
- Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes.Molecular biology of the cell · 1997Article
- Dictyostelium discoideum cells lacking the 34,000-dalton actin-binding protein can grow, locomote, and develop, but exhibit defects in regulation of cell structure and movement: a case of partial redundancy.The Journal of cell biology · 1996Article
- Myristoylated and non-myristoylated forms of the pH sensor protein hisactophilin II: intracellular shuttling to plasma membrane and nucleus monitored in real time by a fusion with green fluorescent protein.The EMBO journal · 1996Article
- Re-expression of ABP-120 rescues cytoskeletal, motility, and phagocytosis defects of ABP-120- Dictyostelium mutants.Molecular biology of the cell · 1996Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin-binding proteins are known to regulate in vitro the assembly of actin into supramolecular structures, but evidence for their activities in living nonmuscle cells is scarce. Amebae of Dictyostelium discoideum are nonmuscle cells in which mutants defective in several actin-binding proteins have been described. Here we characterize a mutant deficient in the 120-kD gelation factor, one of the most abundant F-actin cross-linking proteins of D. discoideum cells. No F-actin cross-linking activity attributable to the 120-kD protein was detected in mutant cell extracts, and antibodies recognizing different epitopes on the polypeptide showed the entire protein was lacking. Under the conditions used, elimination of the gelation factor did not substantially alter growth, shape, motility, or chemotactic orientation of the cells towards a cAMP source. Aggregates of the mutant developed into fruiting bodies consisting of normally differentiated spores and stalk cells. In cytoskeleton preparations a dense network of actin filaments as typical of the cell cortex, and bundles as they extend along the axis of filopods, were recognized. A significant alteration found was an enhanced accumulation of actin in cytoskeletons of the mutant when cells were stimulated with cyclic AMP. Our results indicate that control of cell shape and motility does not require the fine-tuned interactions of all proteins that have been identified as actin-binding proteins by in vitro assays.
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