ArticleThe British journal of ophthalmology2007
Cultured human ocular surface epithelium on therapeutic contact lenses.
Article in The British journal of ophthalmology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 69 citations in OpenAlex.
- Clinical outcomes of xeno-free expansion and transplantation of autologous ocular surface epithelial stem cells via contact lens delivery: a prospective case series.Stem cell research & therapy · 2015Trial
- Goals and Challenges of Stem Cell-Based Therapy for Corneal Blindness Due to Limbal Deficiency.Pharmaceutics · 2021Review
- Bioengineering Approaches for Corneal Regenerative Medicine.Tissue engineering and regenerative medicine · 2020Review
- Clinical Trials of Limbal Stem Cell Deficiency Treated with Oral Mucosal Epithelial Cells.International journal of molecular sciences · 2020Review
- Levels of oxidative DNA damage are low in ex vivo engineered human limbal epithelial tissue.Acta ophthalmologica · 2018Article
- Safety of Cultivated Limbal Epithelial Stem Cell Transplantation for Human Corneal Regeneration.Stem cells international · 2017Review
- Limbal Stem Cell Deficiency: Current Treatment Options and Emerging Therapies.Stem cells international · 2016Review
- Pre-Clinical Cell-Based Therapy for Limbal Stem Cell Deficiency.Journal of functional biomaterials · 2015Review
- Synthetic vs natural scaffolds for human limbal stem cells.Croatian medical journal · 2015Article
- Stem cell-based therapy for treating limbal stem cells deficiency: A review of different strategies.Saudi journal of ophthalmology : official journal of the Saudi Ophthalmological Society · 2014Article
- Application of adipose-derived stem cells on scleral contact lens carrier in an animal model of severe acute alkaline burn.Eye & contact lens · 2014Article
- Plasma polymer-coated contact lenses for the culture and transfer of corneal epithelial cells in the treatment of limbal stem cell deficiency.Tissue engineering. Part A · 2014Article
- Mesenchymal stem cells, nanofiber scaffolds and ocular surface reconstruction.Stem cell reviews and reports · 2013Article
- Growth of corneal epithelial cells over in situ therapeutic contact lens after simple limbal epithelial transplantation (SLET).BMJ case reports · 2013Article
- Towards the use of hydrogels in the treatment of limbal stem cell deficiency.Drug discovery today · 2013Review
- Tissue-regenerating, vision-restoring corneal epithelial stem cells.Stem cell reviews and reports · 2011Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThis study was initiated after observation of some intriguing epithelial growth properties of contact lenses used as a bandage for patients after pterygium surgery.
aimTo determine the efficacy of culturing human ocular surface epithelial cells on therapeutic contact lenses in autologous serum with a view of using this system to transfer epithelial cells to patients with persistent corneal or limbal defects.
methodsExcess graft tissue resected from patients undergoing pterygium surgery (n = 3) consisting of limbal epithelium was placed on siloxane-hydrogel contact lenses (lotrafilcon A and balafilcon A). Limbal explants were cultured in media with 10% autologous serum. Morphology, proliferative capacity and cytokeratin profile were determined by phase contrast, light and electron microscopy, and immunohistochemical analysis.
resultsLotrafilcon A contact lenses sustained proliferation and migration from limbal tissue. Cells became confluent after 10-14 days and consisted of 2-3 layers with a corneal phenotype (CK3(+)/CK12(+)/CK19(-)) and a propensity to proliferate (p63(+)). Electron microscopy showed microvilli on the apical surface with adhesive projections, indicating that these cells were stable and likely to survive for a long term. Growth was not observed from limbal explants cultured on balafilcon A contact lenses.
conclusionA method for culturing human ocular surface epithelium on contact lenses that may facilitate expansion and transfer of autologous limbal epithelial cells while avoiding the risks associated with transplanting allogeneic tissue has been developed. This technique may be potentially useful for the treatment of patients with limbal stem cell deficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.