ArticleThe Biochemical journal2007
Yeast sphingolipids do not need to contain very long chain fatty acids.
Article in The Biochemical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Optical control of sphingolipid biosynthesis using photoswitchable sphingosines.Journal of lipid research · 2025Article
- Optical control of sphingolipid biosynthesis using photoswitchable sphingosines.bioRxiv : the preprint server for biology · 2024Article
- Modifying the yeast very long chain fatty acid biosynthetic machinery by the expression of plant 3-ketoacyl CoA synthase isozymes.Scientific reports · 2022Article
- Article
- Mapping bilayer thickness in the ER membrane.Science advances · 2020Article
- Chemogenetic E-MAP in Saccharomyces cerevisiae for Identification of Membrane Transporters Operating Lipid Flip Flop.PLoS genetics · 2016Article
- Budding Yeast: An Ideal Backdrop forFrontiers in cell and developmental biology · 2016Review
- Dual functions of the trans-2-enoyl-CoA reductase TER in the sphingosine 1-phosphate metabolic pathway and in fatty acid elongation.The Journal of biological chemistry · 2014Article
- The yeast sphingolipid signaling landscape.Chemistry and physics of lipids · 2014Review
- Control of glucosylceramide production and morphogenesis by the Bar1 ceramide synthase in Fusarium graminearum.PloS one · 2011Article
- Two pathways of sphingolipid biosynthesis are separated in the yeast Pichia pastoris.The Journal of biological chemistry · 2011Article
- Yeast cells lacking all known ceramide synthases continue to make complex sphingolipids and to incorporate ceramides into glycosylphosphatidylinositol (GPI) anchors.The Journal of biological chemistry · 2011Article
- Mammalian ceramide synthases.IUBMB life · 2010Review
- Roles for sphingolipids in Saccharomyces cerevisiae.Advances in experimental medicine and biology · 2010Review
- Sphingomyelin synthase SMS2 displays dual activity as ceramide phosphoethanolamine synthase.Journal of lipid research · 2009Article
- Functional characterization of the Arabidopsis beta-ketoacyl-coenzyme A reductase candidates of the fatty acid elongase.Plant physiology · 2009Article
- Sphingomyelin synthase-related protein SMSr controls ceramide homeostasis in the ER.The Journal of cell biology · 2009Article
- Incorporation of ceramides into Saccharomyces cerevisiae glycosylphosphatidylinositol-anchored proteins can be monitored in vitro.Eukaryotic cell · 2009Article
- Thematic review series: sphingolipids. New insights into sphingolipid metabolism and function in budding yeast.Journal of lipid research · 2008Review
- Differential expression of (dihydro)ceramide synthases in mouse brain: oligodendrocyte-specific expression of CerS2/Lass2.Histochemistry and cell biology · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthesis of VLCFAs (very long chain fatty acids) and biosynthesis of DHS (dihydrosphingosine) both are of vital importance for Saccharomyces cerevisiae. The bulk of VLCFAs and DHS are used for ceramide synthesis by the Lag1p (longevity-assurance gene 1)/Lac1p (longevity-assurance gene cognate 1)/Lip1p (Lag1p/Lac1p interacting protein) ceramide synthase. LAG1 and LAC1 are redundant but LIP1 is essential. Here we show that 4Delta (lag1Deltalac1Deltaypc1Deltaydc1Delta) cells devoid of all known endogenous ceramide synthesis pathways are unviable but can be rescued by the expression of Lass5, a mouse LAG1 homologue. Ceramide synthase activity of 4Delta.Lass5 cells only utilizes C16 and C18 fatty acids and does not require the help of Lip1p, an essential cofactor of Lag1p/Lac1p. HPLC-electrospray ionization-MS/MS analysis demonstrated that in IPCs (inositolphosphorylceramides) of 4Delta.Lass5, the very long chain fatty acids (C26 and C24) account for <1% instead of the normal >97%. Notwithstanding, IPCs incorporated into glycosylphosphatidylinositol anchors of 4Delta.Lass5 show normal mobility on TLC and the ceramide- and raft-dependent traffic of Gas1p (glycophospholipid-anchored surface protein) from endoplasmic reticulum to Golgi remains almost normal. Moreover, the biosynthesis of C24:0 fatty acids remains essential. Thus, C(24:0) and dihydrosphingosine are both necessary for survival of yeast cells even if they utilize C16 and C18 fatty acids for sphingolipid biosynthesis.
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