ArticleThe EMBO journal1990
Three distinct ribonucleoproteins from tobacco chloroplasts: each contains a unique amino terminal acidic domain and two ribonucleoprotein consensus motifs.
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 138 citations in OpenAlex.
- The Chloroplast RNA Binding Protein CP31A Has a Preference for mRNAs Encoding the Subunits of the Chloroplast NAD(P)H Dehydrogenase Complex and Is Required for Their Accumulation.International journal of molecular sciences · 2020Article
- The Chloroplast Ribonucleoprotein CP33B Quantitatively Binds thePlants (Basel, Switzerland) · 2020Article
- Flower senescence: some molecular aspects.Planta · 2014Review
- A novel RNA binding protein affects rbcL gene expression and is specific to bundle sheath chloroplasts in C4 plants.BMC plant biology · 2013Article
- Arabidopsis chloroplast RNA binding proteins CP31A and CP29A associate with large transcript pools and confer cold stress tolerance by influencing multiple chloroplast RNA processing steps.The Plant cell · 2012Article
- A site-specific factor interacts directly with its cognate RNA editing site in chloroplast transcripts.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Multiple elements required for translation of plastid atpB mRNA lacking the Shine-Dalgarno sequence.Nucleic acids research · 2004Article
- Endonucleolytic activation directs dark-induced chloroplast mRNA degradation.Nucleic acids research · 2002Article
- A family of RRM-type RNA-binding proteins specific to plant mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Genome analysis: RNA recognition motif (RRM) and K homology (KH) domain RNA-binding proteins from the flowering plant Arabidopsis thaliana.Nucleic acids research · 2002Article
- Involvement of a site-specific trans-acting factor and a common RNA-binding protein in the editing of chloroplast mRNAs: development of a chloroplast in vitro RNA editing system.The EMBO journal · 2001Article
- The sequence and secondary structure of the 3'-UTR affect 3'-end maturation, RNA accumulation, and translation in tobacco chloroplasts.Plant molecular biology · 2000Article
- Characterization of protein-binding to the spinach chloroplast psbA mRNA 5' untranslated region.Nucleic acids research · 1998Article
- Characterization of a gene encoding a DNA-binding protein that interacts in vitro with vascular specific cis elements of the phenylalanine ammonia-lyase promoter.Plant molecular biology · 1997Article
- Differential expression of pathogen-responsive genes encoding two types of glycine-rich proteins in barley.Plant molecular biology · 1997Article
- Regulation of gene expression in chloroplasts of higher plants.Plant molecular biology · 1996Review
- Molecular cloning of a RNA binding protein, S1-1.Nucleic acids research · 1996Article
- A ribosomal protein gene (rpl32) from tobacco chloroplast DNA is transcribed from alternative promoters: similarities in promoter region organization in plastid housekeeping genes.Molecular & general genetics : MGG · 1996Article
- Transfer of rps19 to the nucleus involves the gain of an RNP-binding motif which may functionally replace RPS13 in Arabidopsis mitochondria.The EMBO journal · 1996Article
- Isolation of a novel RNA-binding protein and its association with a large ribonucleoprotein particle present in the nucleoplasm of tobacco cells.Plant molecular biology · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplasts contain their own genetic system. Eighteen different split genes have been found among approximately 130 chloroplast genes from higher plants. However, little is known about the chloroplast splicing system. Mammalian heterogeneous nuclear ribonucleoproteins (hnRNP proteins) have been shown to be involved in splicing. We applied a purification procedure developed for HeLa cell hnRNP proteins, which uses a single-stranded DNA (ssDNA) affinity column, directly to the tobacco chloroplast lysate to isolate their chloroplast counterparts. Four proteins (mol. wt approximately 30 kd) bound strongly to the column. The amino-terminal sequences of three of them were determined and their cDNA clones were isolated from a tobacco leaf cDNA library. Sequence analysis of these clones revealed that all three proteins contain two ribonucleoprotein consensus sequences (RNP-CS), confirming their ribonucleoprotein (RNP) nature. The presence of putative transit peptides in their predicted protein sequences, and an in vitro import experiment confirmed they are located in the chloroplast. This is the first report of organellar proteins containing RNP-CS. In addition, these three chloroplast proteins have a very acidic amino-terminal domain, a novel feature among RNP proteins identified so far. They are expressed both in leaves and roots; their mRNA levels showed different light modulation in mature leaves. The three proteins might be involved in splicing and/or processing of chloroplast RNAs.
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