ArticleScience's STKE : signal transduction knowledge environment2006
Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single cells.
Article in Science's STKE : signal transduction knowledge environment, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed.
- Red-Shifted Epac-Based FRET cAMP Sensors for All-Optical cAMP Control and Multiparameter Imaging.Cells · 2026Article
- Bok regulates mitochondrial fusion and morphology.Cell death and differentiation · 2019Article
- Intracellular cAMP Sensor EPAC: Physiology, Pathophysiology, and Therapeutics Development.Physiological reviews · 2018Review
- Using cAMP Sensors to Study Cardiac Nanodomains.Journal of cardiovascular development and disease · 2018Review
- Stromal Interaction Molecule 1 (STIM1) Regulates ATP-sensitive Potassium (The Journal of biological chemistry · 2017Article
- Glucagon-Like Peptide-1 and Its Class B G Protein-Coupled Receptors: A Long March to Therapeutic Successes.Pharmacological reviews · 2016Review
- GPR119 Agonist AS1269574 Activates TRPA1 Cation Channels to Stimulate GLP-1 Secretion.Molecular endocrinology (Baltimore, Md.) · 2016Article
- The ins and outs of adrenergic signaling.Journal of molecular medicine (Berlin, Germany) · 2015Review
- Article
- New insights concerning the molecular basis for defective glucoregulation in soluble adenylyl cyclase knockout mice.Biochimica et biophysica acta · 2014Review
- cAMP and Ca²⁺ signaling in secretory epithelia: crosstalk and synergism.Cell calcium · 2014Review
- Serum- and glucocorticoid-induced protein kinase 1 (SGK1) is regulated by store-operated Ca2+ entry and mediates cytoprotection against necrotic cell death.The Journal of biological chemistry · 2013Article
- Imaging the coordination of multiple signalling activities in living cells.Nature reviews. Molecular cell biology · 2011Review
- Reporting from the field: genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems.Annual review of biochemistry · 2011Review
- Calcium-dependent increases in protein kinase-A activity in mouse retinal ganglion cells are mediated by multiple adenylate cyclases.PloS one · 2009Article
- Synthetic analogs of FTY720 [2-amino-2-(2-[4-octylphenyl]ethyl)-1,3-propanediol] differentially regulate pulmonary vascular permeability in vivo and in vitro.The Journal of pharmacology and experimental therapeutics · 2009Article
- Enhanced Rap1 activation and insulin secretagogue properties of an acetoxymethyl ester of an Epac-selective cyclic AMP analog in rat INS-1 cells: studies with 8-pCPT-2'-O-Me-cAMP-AM.The Journal of biological chemistry · 2009Article
- Imaging cytoplasmic cAMP in mouse brainstem neurons.BMC neuroscience · 2009Article
- A technique for simultaneous measurement of Ca2+, FRET fluorescence and force in intact mouse small arteries.The Journal of physiology · 2008Article
- Imaging second messenger dynamics in developing neural circuits.Developmental neurobiology · 2008Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Understanding the temporal and spatial integration of the Ca2+ and adenosine 3',5'-monophosphate (cAMP) signaling pathways requires concurrent measurements of both second messengers. Here, we describe an optical technique to simultaneously image cAMP and Ca2+ concentration gradients in MIN6 mouse insulinoma cells using Epac1-camps, a Förster (or fluorescence) resonance energy transfer (FRET)-based cAMP biosensor, and Fura-2, a fluorescent indicator of Ca2+. This real-time imaging method allows investigation of the dynamic organization and integration of multiple levels of signal processing in single living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.