ArticleNucleic acids research2006
The spatial arrangement of ORC binding modules determines the functionality of replication origins in budding yeast.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- INO80C Remodeler Maintains Genomic Stability by Preventing Promiscuous Transcription at Replication Origins.Cell reports · 2020Article
- Order from clutter: selective interactions at mammalian replication origins.Nature reviews. Genetics · 2017Review
- Article
- High-resolution mapping, characterization, and optimization of autonomously replicating sequences in yeast.Genome research · 2013Article
- Altered replication in human cells promotes DMPK (CTG)(n) · (CAG)(n) repeat instability.Molecular and cellular biology · 2012Article
- Analysis of model replication origins in Drosophila reveals new aspects of the chromatin landscape and its relationship to origin activity and the prereplicative complex.Molecular biology of the cell · 2012Article
- High-resolution analysis of four efficient yeast replication origins reveals new insights into the ORC and putative MCM binding elements.Nucleic acids research · 2011Article
- Differential targeting of Tetrahymena ORC to ribosomal DNA and non-rDNA replication origins.The EMBO journal · 2009Article
- Computational detection of significant variation in binding affinity across two sets of sequences with application to the analysis of replication origins in yeast.BMC bioinformatics · 2008Article
- Analysis of chromosome III replicators reveals an unusual structure for the ARS318 silencer origin and a conserved WTW sequence within the origin recognition complex binding site.Molecular and cellular biology · 2008Article
- Cell cycle regulation of DNA replication.Annual review of genetics · 2007Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the quest to define autonomously replicating sequences (ARSs) in eukaryotic cells, an ARS consensus sequence (ACS) has emerged for budding yeast. This ACS is recognized by the replication initiator, the origin recognition complex (ORC). However, not every match to the ACS constitutes a replication origin. Here, we investigated the requirements for ORC binding to origins that carry multiple, redundant ACSs, such as ARS603. Previous studies raised the possibility that these ACSs function as individual ORC binding sites. Detailed mutational analysis of the two ACSs in ARS603 revealed that they function in concert and give rise to an initiation pattern compatible with a single bipartite ORC binding site. Consistent with this notion, deletion of one base pair between the ACS matches abolished ORC binding at ARS603. Importantly, loss of ORC binding in vitro correlated with the loss of ARS activity in vivo. Our results argue that replication origins in yeast are in general comprised of bipartite ORC binding sites that cannot function in random alignment but must conform to a configuration that permits ORC binding. These requirements help to explain why only a limited number of ACS matches in the yeast genome qualify as ORC binding sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.