ArticleThe Biochemical journal2007
The C-terminus of cytochrome b5 confers endoplasmic reticulum specificity by preventing spontaneous insertion into membranes.
Article in The Biochemical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Membrane Protein Insertion in Cells: Principles, Pathways, and Quality Control.Chemical reviews · 2026Review
- A reference library for assigning protein subcellular localizations by image-based machine learning.The Journal of cell biology · 2020Article
- Genome-wide analysis of Homo sapiens, Arabidopsis thaliana, and Saccharomyces cerevisiae reveals novel attributes of tail-anchored membrane proteins.BMC genomics · 2019Article
- Targeting of tail-anchored membrane proteins to subcellular organelles in Toxoplasma gondii.Traffic (Copenhagen, Denmark) · 2017Article
- The N termini of Brassica and tung omega-3 fatty acid desaturases mediate proteasome-dependent protein degradation in plant cells.Plant signaling & behavior · 2011Article
- Tail-anchored membrane proteins: exploring the complex diversity of tail-anchored-protein targeting in plant cells.Plant cell reports · 2011Review
- Distinct pathways mediate the sorting of tail-anchored proteins to the plastid outer envelope.PloS one · 2010Article
- Biogenesis of tail-anchored proteins: the beginning for the end?Journal of cell science · 2009Review
- Hydrophobic profiles of the tail anchors in SLMAP dictate subcellular targeting.BMC cell biology · 2009Article
- Redox compartmentalization in eukaryotic cells.Biochimica et biophysica acta · 2008Review
- Localization of the Carnation Italian ringspot virus replication protein p36 to the mitochondrial outer membrane is mediated by an internal targeting signal and the TOM complex.BMC cell biology · 2008Article
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Authors and funding
5 authors.
Funding
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Abstract
The molecular mechanisms that determine the correct subcellular localization of proteins targeted to membranes by tail-anchor sequences are poorly defined. Previously, we showed that two isoforms of the tung oil tree [Vernicia (Aleurites) fordii] tail-anchored Cb5 (cytochrome b5) target specifically to ER (endoplasmic reticulum) membranes both in vivo and in vitro [Hwang, Pelitire, Henderson, Andrews, Dyer and Mullen (2004) Plant Cell 16, 3002-3019]. In the present study, we examine the targeting of various tung Cb5 fusion proteins and truncation mutants to purified intracellular membranes in vitro in order to assess the importance of the charged CTS (C-terminal sequence) in targeting to specific membranes. Removal of the CTS from tung Cb5 proteins resulted in efficient binding to both ER and mitochondria. Results from organelle competition, liposome-binding and membrane proteolysis experiments demonstrated that removal of the CTS results in spontaneous insertion of tung Cb5 proteins into lipid bilayers. Our results indicate that the CTSs from plant Cb5 proteins provide ER specificity by preventing spontaneous insertion into incorrect subcellular membranes.
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