ArticleProceedings of the National Academy of Sciences of the United States of America2006
Electrostatic interactions in the channel cavity as an important determinant of potassium channel selectivity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Understanding Ion Channel Gating Mechanisms by Molecular Dynamics Simulations.Advances in experimental medicine and biology · 2026Review
- Preorganized Electric Fields in Voltage-Gated Sodium Channels.Chembiochem : a European journal of chemical biology · 2025Article
- Pore-forming transmembrane domains control ion selectivity and selectivity filter conformation in the KirBac1.1 potassium channel.The Journal of general physiology · 2021Article
- TOK channels use the two gates in classical KFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Permeation mechanisms through the selectivity filter and the open helix bundle crossing gate of GIRK2.Computational and structural biotechnology journal · 2020Article
- A novel mutation KCNQ1p.Thr312del is responsible for long QT syndrome type 1.Heart and vessels · 2019Article
- Prolonged seizure activity causes caspase dependent cleavage and dysfunction of G-protein activated inwardly rectifying potassium channels.Scientific reports · 2017Article
- Structure-guided transformation of channelrhodopsin into a light-activated chloride channel.Science (New York, N.Y.) · 2014Article
- Effect of diaminopropionic acid (Dap) on the biophysical properties of a modified synthetic channel-forming peptide.Molecular pharmaceutics · 2013Article
- Structural correlates of selectivity and inactivation in potassium channels.Biochimica et biophysica acta · 2012Review
- Architecture of the HCN selectivity filter and control of cation permeation.Scientific reports · 2012Article
- Mechanism of potassium-channel selectivity revealed by Na(+) and Li(+) binding sites within the KcsA pore.Nature structural & molecular biology · 2009Article
- Changes in negative charge at the luminal mouth of the pore alter ion handling and gating in the cardiac ryanodine-receptor.Biophysical journal · 2009Article
- Model development for the viral Kcv potassium channel.Biophysical journal · 2009Article
- Long-pore electrostatics in inward-rectifier potassium channels.The Journal of general physiology · 2008Article
- The membrane potential and its representation by a constant electric field in computer simulations.Biophysical journal · 2008Article
- Tl+-induced micros gating of current indicates instability of the MaxiK selectivity filter as caused by ion/pore interaction.The Journal of general physiology · 2008Article
- K+/Na+ selectivity in K channels and valinomycin: over-coordination versus cavity-size constraints.Journal of molecular biology · 2008Article
- Tuning ion coordination architectures to enable selective partitioning.Biophysical journal · 2007Article
- Ion conductance vs. pore gating and selectivity in KcsA channel: modeling achievements and perspectives.Journal of molecular modeling · 2007Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Potassium channels are membrane proteins that allow the passage of potassium ions at near diffusion rates while severely limiting the flux of the slightly smaller sodium ions. Although studies thus far have focused on the narrowest part of the channel, known as the selectivity filter, channels are long pores with multiple ions that traverse the selectivity filter, the water-filled central cavity, and the rest of the pore formed by cytoplasmic domains. Here, we present experimental analyses on Kir3.2 (GIRK2), a G protein-activated inwardly rectifying potassium (Kir) channel, showing that a negative charge introduced at a pore-facing position in the cavity (N184) below the selectivity filter restores both K(+) selectivity and inward rectification properties to the nonselective S177W mutant channel. Molecular modeling demonstrates that the negative residue has no effect on the geometry of the selectivity filter, suggesting that it has a local effect on the cavity ion. Moreover, restoration of selectivity does not depend on the exact location of the charge in the central cavity as long as this residue faces the pore, where it is in close contact with permeant ions. Our results indicate that interactions between permeant ions and the channel cavity can influence ion selectivity and channel block by means of an electrostatic effect.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.