ArticleMolecular and cellular biology2006
Specific isoforms of translation initiation factor 4GI show differences in translational activity.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- HNRNPH1-mediated splicing events regulatebioRxiv : the preprint server for biology · 2025Article
- Ribosome profiling reveals a functional role for autophagy in mRNA translational control.Communications biology · 2020Article
- A threonyl-tRNA synthetase-mediated translation initiation machinery.Nature communications · 2019Article
- O-GlcNAc modification of eIF4GI acts as a translational switch in heat shock response.Nature chemical biology · 2018Article
- Norovirus translation requires an interaction between the C Terminus of the genome-linked viral protein VPg and eukaryotic translation initiation factor 4G.The Journal of biological chemistry · 2014Article
- mTOR inhibition and levels of the DNA repair protein MGMT in T98G glioblastoma cells.Molecular cancer · 2014Article
- Role of translation initiation factor 4G in lifespan regulation and age-related health.Ageing research reviews · 2014Review
- mTOR kinase-dependent, but raptor-independent regulation of downstream signaling is important for cell cycle exit and myogenic differentiation.Cell cycle (Georgetown, Tex.) · 2014Article
- Eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA decay by two genetically separable mechanisms.PloS one · 2014Article
- Phosphorylation of eIF4GII and 4E-BP1 in response to nocodazole treatment: a reappraisal of translation initiation during mitosis.Cell cycle (Georgetown, Tex.) · 2013Article
- Reduced DEAF1 function during type 1 diabetes inhibits translation in lymph node stromal cells by suppressing Eif4g3.Journal of molecular cell biology · 2013Article
- SLiMPrints: conservation-based discovery of functional motif fingerprints in intrinsically disordered protein regions.Nucleic acids research · 2012Article
- Proteomic and protein interaction network analysis of human T lymphocytes during cell-cycle entry.Molecular systems biology · 2012Article
- Molecular mechanism of scanning and start codon selection in eukaryotes.Microbiology and molecular biology reviews : MMBR · 2011Review
- Inhibition of the interactions between eukaryotic initiation factors 4E and 4G impairs long-term associative memory consolidation but not reconsolidation.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The multifaceted poliovirus 2A protease: regulation of gene expression by picornavirus proteases.Journal of biomedicine & biotechnology · 2011Article
- Noncytotoxic inhibition of viral infection through eIF4F-independent suppression of translation by 4EGi-1.Journal of virology · 2011Article
- Deletion of the eIFiso4G subunit of the Arabidopsis eIFiso4F translation initiation complex impairs health and viability.Plant molecular biology · 2010Article
- Article
- Design of RNAi hairpins for mutation-specific silencing of ataxin-7 and correction of a SCA7 phenotype.PloS one · 2009Article
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The eukaryotic initiation factor (eIF) 4GI gene locus (eIF4GI) contains three identified promoters, generating alternately spliced mRNAs, yielding a total of five eIF4GI protein isoforms. Although eIF4GI plays a critical role in mRNA recruitment to the ribosomes, little is known about the functions of the different isoforms, their partner binding capacities, or the role of the homolog, eIF4GII, in translation initiation. To directly address this, we have used short interfering RNAs (siRNAs) expressed from DNA vectors to silence the expression of eIF4GI in HeLa cells. Here we show that reduced levels of specific mRNA and eIF4GI isoforms in HeLa cells promoted aberrant morphology and a partial inhibition of translation. The latter reflected dephosphorylation of 4E-BP1 and decreased eIF4F complex levels, with no change in eIF2alpha phosphorylation. Expression of siRNA-resistant Myc-tagged eIF4GI isoforms has allowed us to show that the different isoforms exhibit significant differences in their ability to restore translation rates. Here we quantify the efficiency of eIF4GI promoter usage in mammalian cells and demonstrate that even though the longest isoform of eIF4GI (eIF4GIf) was relatively poorly expressed when reintroduced, it was more efficient at promoting the translation of cellular mRNAs than the more highly expressed shorter isoforms used in previous functional studies.
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