ArticleJournal of bacteriology2006
Rem, a new transcriptional activator of motility and chemotaxis in Sinorhizobium meliloti.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 82 citations in OpenAlex.
- A clarifying perspective on bacterial pseudo-receiver domains.Journal of bacteriology · 2025Review
- The motility and chemosensory systems of Rhizobium leguminosarum, their role in symbiosis, and link to PTSEnvironmental microbiology · 2024Article
- Chromosomal gene of hybrid multisensor histidine kinase is involved in motility regulation in the rhizobacterium Azospirillum baldaniorum Sp245 under mechanical and water stress.World journal of microbiology & biotechnology · 2023Article
- MucR fromInternational journal of molecular sciences · 2023Article
- A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum.PloS one · 2023Article
- Activation of ChvG-ChvI regulon by cell wall stress confers resistance to β-lactam antibiotics and initiates surface spreading in Agrobacterium tumefaciens.PLoS genetics · 2022Article
- FliL and its paralog MotF have distinct roles in the stator activity of the Sinorhizobium meliloti flagellar motor.Molecular microbiology · 2022Article
- Flagellin outer domain dimerization modulates motility in pathogenic and soil bacteria from viscous environments.Nature communications · 2022Article
- The zinc-finger bearing xenogeneic silencer MucR in α-proteobacteria balances adaptation and regulatory integrity.The ISME journal · 2022Article
- Influence of host genotype in establishing root associated microbiome ofFrontiers in microbiology · 2022Article
- Motility control through an anti-activation mechanism in Agrobacterium tumefaciens.Molecular microbiology · 2021Article
- Dual Control of Flagellar Synthesis and Exopolysaccharide Production by FlbD-FliX Class II Regulatory Proteins in Bradyrhizobium diazoefficiens.Journal of bacteriology · 2021Article
- Rhizobial Chemotaxis and Motility Systems at Work in the Soil.Frontiers in plant science · 2021Review
- Programmed Proteolysis of Chemotaxis Proteins in Sinorhizobium meliloti: Features in the C-Terminal Region Control McpU Degradation.Journal of bacteriology · 2020Article
- Cellular Stoichiometry of Chemotaxis Proteins inJournal of bacteriology · 2020Article
- An ECF41 family σ factor controls motility and biogenesis of lateral flagella inJournal of bacteriology · 2020Article
- A Novel OmpR-Type Response Regulator Controls Multiple Stages of theFrontiers in microbiology · 2020Article
- An Updated Perspective onFrontiers in microbiology · 2020Article
- A high-throughput system to identify inhibitors ofProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Genome-wide identification of genes directly regulated by ChvI and a consensus sequence for ChvI binding in Sinorhizobium meliloti.Molecular microbiology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of 51 known genes clustered in the flagellar regulon of Sinorhizobium meliloti is organized as a three-class hierarchy: class IA comprises the master regulatory genes, visN and visR; class II, controlled by VisNR, comprises flagellar assembly and motility genes; and class III comprises flagellin and chemotaxis genes requiring class II for expression. The expression of visN-visR is constitutive throughout growth, whereas that of class II and class III genes is limited to exponential growth. A new OmpR-like, 25-kDa transcription factor, Rem, whose synthesis is confined to exponential growth, was shown to positively control swimming motility. No phosphorylation of the receiver domain of Rem was required for its activity. Gene expression in tester strains with known deficiencies placed the rem gene (class IB) below visN-visR (class IA) and above class II genes in the regulatory cascade. Footprinting analysis demonstrated that the Rem protein binds to class II gene promoters as well as to its own promoter, indicating that this protein is autoregulatory. An alignment of the Rem-protected DNA sequences revealed a conserved binding motif of imperfect tandem repeats overlapping a predicted -35 promoter box by 3 bp. This new promoter was confirmed by mapping the transcription start site of a typical class II gene, flgB, 5 nucleotides downstream of the -10 promoter box. The transcription of rem is under dual control of an upstream (Rem-activated) class II-type promoter and a downstream (VisNR-activated) sigma70-like promoter. The central role of Rem as the growth-dependent transcriptional activator intermediate between the master regulator, VisNR, and the flagellar and motility genes is a new distinguishing feature of the S. meliloti regulatory cascade.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.