ArticleJournal of bacteriology2006
The TadV protein of Actinobacillus actinomycetemcomitans is a novel aspartic acid prepilin peptidase required for maturation of the Flp1 pilin and TadE and TadF pseudopilins.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed.
- Genomic Insights intoPathogens (Basel, Switzerland) · 2025Article
- Electron Cryo-Tomography Reveals thebioRxiv : the preprint server for biology · 2025Article
- The structure of the Tad pilus alignment complex reveals a periplasmic conduit for pilus extension.Nature communications · 2025Article
- Tad pili with adaptable tips mediate contact-dependent killing during bacterial predation.Nature communications · 2025Article
- Structural mechanisms of Tad pilus assembly and its interaction with an RNA virus.Science advances · 2024Article
- Article
- Assembly mechanism of a Tad secretion system secretin-pilotin complex.Nature communications · 2023Article
- Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask).Microbiology (Reading, England) · 2023Review
- Differential Virulence of Aggregatibacter actinomycetemcomitans Serotypes Explained by Exoproteome Heterogeneity.Microbiology spectrum · 2023Article
- Mutations Suppressing the Lack of Prepilin Peptidase Provide Insights Into the Maturation of the Major Pilin Protein in Cyanobacteria.Frontiers in microbiology · 2021Article
- The type IV pilin PilA couples surface attachment and cell-cycle initiation inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Phosphorylcholine is located in Aggregatibacter actinomycetemcomitans fimbrial protein Flp 1.Medical microbiology and immunology · 2018Article
- Evolution of a Biomass-Fermenting Bacterium To Resist Lignin Phenolics.Applied and environmental microbiology · 2017Article
- Cloning-independent markerless gene editing in Streptococcus sanguinis: novel insights in type IV pilus biology.Nucleic acids research · 2017Article
- Prevalence of Flp Pili-Encoding Plasmids inFrontiers in microbiology · 2017Article
- Novel Pelagic Iron-Oxidizing Zetaproteobacteria from the Chesapeake Bay Oxic-Anoxic Transition Zone.Frontiers in microbiology · 2017Article
- Comparative transcriptomic analysis ofNPJ biofilms and microbiomes · 2017Article
- Inner-membrane protein MorC is involved in fimbriae production and biofilm formation in Aggregatibacter actinomycetemcomitans.Microbiology (Reading, England) · 2016Article
- Identification of putative adhesins of Actinobacillus suis and their homologues in other members of the family Pasteurellaceae.BMC research notes · 2015Article
- Adhesins Involved in Attachment to Abiotic Surfaces by Gram-Negative Bacteria.Microbiology spectrum · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The tad locus of Actinobacillus actinomycetemcomitans encodes genes for the biogenesis of Flp pili, which allow the bacterium to adhere tenaciously to surfaces and form strong biofilms. Although tad (tight adherence) loci are widespread among bacterial and archaeal species, very little is known about the functions of the individual components of the Tad secretion apparatus. Here we characterize the mechanism by which the pre-Flp1 prepilin is processed to the mature pilus subunit. We demonstrate that the tadV gene encodes a prepilin peptidase that is both necessary and sufficient for proteolytic maturation of Flp1. TadV was also found to be required for maturation of the TadE and TadF pilin-like proteins, which we term pseudopilins. Using site-directed mutagenesis, we show that processing of pre-Flp1, pre-TadE, and pre-TadF is required for biofilm formation. Mutation of a highly conserved glutamic acid residue at position +5 of Flp1, relative to the cleavage site, resulted in a processed pilin that was blocked in assembly. In contrast, identical mutations in TadE or TadF had no effect on biofilm formation, indicating that the mechanisms by which Flp1 pilin and the pseudopilins function are distinct. We also determined that two conserved aspartic acid residues in TadV are critical for function of the prepilin peptidase. Together, our results indicate that the A. actinomycetemcomitans TadV protein is a member of a novel subclass of nonmethylating aspartic acid prepilin peptidases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.