ArticleJournal of bacteriology2006
Biochemical and molecular characterization of the biosynthesis of glutamine and glutamate, two major compatible solutes in the moderately halophilic bacterium Halobacillus halophilus.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 70 citations in OpenAlex.
- Application potential of a marine-derived halotolerant yeast in saline azo dye wastewater treatment: elucidating degradation pathways and osmo-adaptive strategies.Bioprocess and biosystems engineering · 2026Article
- Unraveling the halotolerant degradative machinery of Candida tropicalis JH: a novel biocatalyst for sustainable treatment of hypersaline azo dye effluents.World journal of microbiology & biotechnology · 2026Article
- HadS, a membrane-associated 2-haloacid dehalogenase from the moderate halophileFrontiers in microbiology · 2026Article
- Genomic prediction of phytase potential and stress tolerance in maize-associated plant growth-promoting rhizobacterium Enterobacter cloacae Mz49.AMB Express · 2025Article
- Biodegradation of S-Triazine Herbicides Under Saline Conditions byMicroorganisms · 2025Article
- Association Analysis of the Genomic and Functional Characteristics of HalotolerantMicroorganisms · 2025Article
- Bacterial osmoprotectants-a way to survive in saline conditions and potential crop allies.FEMS microbiology reviews · 2025Review
- Whole-Genome Profiling of Endophytic Strain B.L.Ns.14 fromMicroorganisms · 2024Article
- Unveiling Agricultural Biotechnological Prospects: The Draft Genome Sequence of Stenotrophomonas geniculata LGMB417.Current microbiology · 2024Article
- Metabolic rewiring enables ammonium assimilation via a non-canonical fumarate-based pathway.Microbial biotechnology · 2024Article
- Genomic and phenotypic characterization of a red-pigmented strain ofFrontiers in microbiology · 2023Article
- A Second Gamma-Glutamylpolyamine Synthetase, GlnA2, Is Involved in Polyamine Catabolism inInternational journal of molecular sciences · 2022Article
- Salt tolerance-based niche differentiation of soil ammonia oxidizers.The ISME journal · 2022Article
- Insights from comparative proteomic analysis into degradation of phenanthrene and salt tolerance by the halophilic Martelella strain AD-3.Ecotoxicology (London, England) · 2021Article
- Revealing the salinity adaptation mechanism in halotolerant bacterium Egicoccus halophilus EGI 80432Applied microbiology and biotechnology · 2021Article
- Comparative genomics analysis of Nitriliruptoria reveals the genomic differences and salt adaptation strategies.Extremophiles : life under extreme conditions · 2020Article
- Article
- Distinct Osmoadaptation Strategies in the Strict Halophilic and Halotolerant Bacteria Isolated from Lunsu Salt Water Body of North West Himalayas.Current microbiology · 2018Article
- Draft Genome Sequence of Bacillus urumqiensis BZ-SZ-XJ18Genome announcements · 2018Article
- Perspective of ions and messengers: an intricate link between potassium, glutamate, and cyclic di-AMP.Current genetics · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The moderately halophilic, chloride-dependent bacterium Halobacillus halophilus produces glutamate and glutamine as main compatible solutes at external salinities of 1.0 to 1.5 M NaCl. The routes for the biosynthesis of these solutes and their regulation were examined. The genome contains two genes potentially encoding glutamate dehydrogenases and two genes for the small subunit of a glutamate synthase, but only one gene for the large subunit. However, the expression of these genes was not salt dependent, nor were the corresponding enzymatic activities detectable in cell extracts of cells grown at different salinities. In contrast, glutamine synthetase activity was readily detectable in H. halophilus. Induction of glutamine synthetase activity was strictly salt dependent and reached a maximum at 3.0 M NaCl; chloride stimulated the production of active enzyme by about 300%. Two potential genes encoding a glutamine synthetase, glnA1 and glnA2, were identified. The expression of glnA2 but not of glnA1 was increased up to fourfold in cells adapted to high salt, indicating that GlnA2 is the glutamine synthetase involved in the synthesis of the solutes glutamate and glutamine. Furthermore, expression of glnA2 was stimulated twofold by the presence of chloride ions. Chloride exerted an even more pronounced effect on the enzymatic activity of preformed enzyme: in the absence of chloride in the assay buffer, glutamine synthetase activity was decreased by as much as 90%. These data demonstrate for the first time a regulatory role of a component of common salt, chloride, in the biosynthesis of compatible solutes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.