ArticleJournal of bacteriology2006
A recA null mutation may be generated in Streptomyces coelicolor.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Genome-Wide Identification of the LexA-Mediated DNA Damage Response in Streptomyces venezuelae.Journal of bacteriology · 2022Article
- Genome plasticity is governed by double strand break DNA repair in Streptomyces.Scientific reports · 2018Article
- The evolution of no-cost resistance at sub-MIC concentrations of streptomycin in Streptomyces coelicolor.The ISME journal · 2017Article
- Multiple and Variable NHEJ-Like Genes Are Involved in Resistance to DNA Damage inFrontiers in microbiology · 2016Article
- The adnAB locus, encoding a putative helicase-nuclease activity, is essential in Streptomyces.Journal of bacteriology · 2014Article
- Topoisomerase IV is required for partitioning of circular chromosomes but not linear chromosomes in Streptomyces.Nucleic acids research · 2013Article
- A novel docking domain interface model predicting recombination between homoeologous modular biosynthetic gene clusters.Journal of industrial microbiology & biotechnology · 2011Article
- Linear Streptomyces plasmids form superhelical circles through interactions between their terminal proteins.Nucleic acids research · 2011Article
- DNA polymerase I is not required for replication of linear chromosomes in streptomyces.Journal of bacteriology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recombinase RecA plays a crucial role in homologous recombination and the SOS response in bacteria. Although recA mutants usually are defective in homologous recombination and grow poorly, they nevertheless can be isolated in almost all bacteria. Previously, considerable difficulties were experienced by several laboratories in generating recA null mutations in Streptomyces, and the only recA null mutants isolated (from Streptomyces lividans) appeared to be accompanied by a suppressing mutation. Using gene replacement mediated by Escherichia coli-Streptomyces conjugation, we generated recA null mutations in a series of Streptomyces coelicolor A3(2) strains. These recA mutants were very sensitive to mitomycin C but only moderately sensitive to UV irradiation, and the UV survival curves showed wide shoulders, reflecting the presence of a recA-independent repair pathway. The mutants segregated minute colonies with low viability during growth and produced more anucleate spores than the wild type. Some crosses between pairs of recA null mutants generated no detectable recombinants, showing for the first time that conjugal recombination in S. coelicolor is recA mediated, but other mutants retained the ability to undergo recombination. The nature of this novel recombination activity is unknown.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.