ArticleEukaryotic cell2006
Effect of sequence-directed nucleosome disruption on cell-type-specific repression by alpha2/Mcm1 in the yeast genome.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Cell-internal autocrine receptor inactivation supports maintenance of mating-type identity in yeast.Science advances · 2026Article
- Chemical map-based prediction of nucleosome positioning using the Bioconductor package nuCpos.BMC bioinformatics · 2021Article
- Article
- Article
- Sequence-directed nucleosome-depletion is sufficient to activate transcription from a yeast core promoter in vivo.Biochemical and biophysical research communications · 2016Article
- The Scc2-Scc4 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions.Nature genetics · 2014Article
- Telomeric repeats act as nucleosome-disfavouring sequences in vivo.Nucleic acids research · 2014Article
- Archaeal nucleosome positioning in vivo and in vitro is directed by primary sequence motifs.BMC genomics · 2013Article
- The MAT locus genes play different roles in sexual reproduction and pathogenesis in Fusarium graminearum.PloS one · 2013Article
- Quantitative comparison between poly(L-arginine) and poly(L-lysine) at each step of polyplex-based gene transfection using a microinjection technique.Science and technology of advanced materials · 2012Article
- GAL1-SceI directed site-specific genomic (gsSSG) mutagenesis: a method for precisely targeting point mutations in S. cerevisiae.BMC biotechnology · 2011Article
- Shields up: the Tup1-Cyc8 repressor complex blocks coactivator recruitment.Genes & development · 2011Article
- Article
- Distinct modes of regulation by chromatin encoded through nucleosome positioning signals.PLoS computational biology · 2008Article
- Transcriptional repression by the Pho4 transcription factor controls the timing of SNZ1 expression.Eukaryotic cell · 2008Article
- A nucleosome positioned by alpha2/Mcm1 prevents Hap1 activator binding in vivo.Biochemical and biophysical research communications · 2007Article
- Special type of pheromone-induced invasive growth in Saccharomyces cerevisiae.Current genetics · 2007Article
- Exposing the core promoter is sufficient to activate transcription and alter coactivator requirement at RNR3.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
In Saccharomyces cerevisiae, a-cell-specific genes are repressed in MATalpha cells by alpha2/Mcm1, acting in concert with the Ssn6-Tup1 corepressors and the Isw2 chromatin remodeling complex, and nucleosome positioning has been proposed as one mechanism of repression. However, prior studies showed that nucleosome positioning is not essential for repression by alpha2/Mcm1 in artificial reporter plasmids, and the importance of the nucleosome positioning remains questionable. We have tested the function of positioned nucleosomes through alteration of genomic chromatin at the a-cell-specific gene BAR1. We report here that a positioned nucleosome in the BAR1 promoter is disrupted in cis by the insertion of diverse DNA sequences such as poly(dA) . poly(dT) and poly(dC-dG) . poly(dC-dG), leading to inappropriate partial derepression of BAR1. Also, we show that isw2 mutation causes loss of nucleosome positioning in BAR1 in MATalpha cells as well as partial disruption of repression. Thus, nucleosome positioning is required for full repression, but loss of nucleosome positioning is not sufficient to relieve repression completely. Even though disruption of nucleosome positioning by the cis- and trans-acting modulators of chromatin has a modest effect on the level of transcription, it causes significant degradation of the alpha-mating pheromone in MATalpha cells, thereby affecting its cell type identity. Our results illustrate a useful paradigm for analysis of chromatin structural effects at genomic loci.
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