ArticlePlanta2007
Identification and characterization of genes expressed in early embryogenesis from microspores of Brassica napus.
Article in Planta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 77 citations in OpenAlex.
- Molecular Insights into Microspore Embryogenesis: Transcriptomic Dynamics in Barley and Wheat.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Transcriptomic identification of potential antioxidative enzyme regulators of the gametophytic-to-embryogenic switch in barley microspores.Frontiers in plant science · 2025Article
- The Research Process of PSK Biosynthesis, Signaling Transduction, and Potential Applications inPlants (Basel, Switzerland) · 2023Review
- Review
- Effects of genotype and culture conditions on microspore embryogenesis in radish (Molecular breeding : new strategies in plant improvement · 2022Article
- The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents.Frontiers in plant science · 2021Article
- Global Transcriptome and Coexpression Network Analyses Reveal New Insights Into Somatic Embryogenesis in Hybrid Sweetgum (Frontiers in plant science · 2021Article
- Transcriptomic analysis reveals somatic embryogenesis-associated signaling pathways and gene expression regulation in maize (Zea mays L.).Plant molecular biology · 2020Article
- Identification, Gene Structure, and Expression ofFrontiers in plant science · 2020Article
- Comparative gene expression profile analysis of ovules provides insights into Jatropha curcas L. ovule development.Scientific reports · 2019Article
- Genetic Loci Governing Androgenic Capacity in Perennial Ryegrass (G3 (Bethesda, Md.) · 2018Article
- Identification of loci associated with embryo yield in microspore culture of Brassica rapa by segregation distortion analysis.Plant cell reports · 2016Article
- Transcriptional identification and characterization of differentially expressed genes associated with embryogenesis in radish (Raphanus sativus L.).Scientific reports · 2016Article
- Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration.Plant cell reports · 2016Article
- Isolation of differentially expressed sex genes in garden asparagus using suppression subtractive hybridization.Journal of plant research · 2015Article
- Label-Free Quantitative Proteomics of Embryogenic and Non-Embryogenic Callus during Sugarcane Somatic Embryogenesis.PloS one · 2015Article
- Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association.Frontiers in plant science · 2015Article
- Instability in mitochondrial membranes in Polima cytoplasmic male sterility of Brassica rapa ssp. chinensis.Functional & integrative genomics · 2014Article
- Microspore embryogenesis in wheat: new marker genes for early, middle and late stages of embryo development.Plant reproduction · 2013Article
- Microspore embryogenesis: establishment of embryo identity and pattern in culture.Plant reproduction · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the mechanism in induction of embryogenesis from microspores of Brassica napus, we isolated exhaustively the genes expressed differentially during the early stage of microspore culture. A subtracted cDNA library composed of up-regulated genes during androgenic initiation was produced by suppression subtractive hybridization followed by differential screening by dot blot hybridization, and a total of 136 non-redundant expressed sequence tags were identified. Analysis of the potential functions of the genes showed that 64% of these genes were homologous to known genes, and the remaining ones have not been previously reported to participate in embryogenesis. Many embryo-specific genes were contained in the isolated genes, for example, genes cording lipid transfer protein, napin, cruciferin, oleosin, and phytosulfokine. Real-time RT-PCR analysis for 15 selected genes, which are understood to not be related with embryogenesis, demonstrated that all genes were expressed highly in the early stage of microspore embryogenesis. A few genes also showed higher expression in microspores cultured in non-embryogenic condition or in later stages of embryos. A principal component analysis based on expression profiles of the 15 genes demonstrated that these genes were classified into 2 groups, one characterized by their high expression in initiation of embryogenesis, and the other characterized by their expression in the early to middle stage of embryogenesis. The expressions of these genes were confirmed in zygotic embryos. The identification and characterization of the genes isolated in the present study provide novel information on microspore embryogenesis in Brassica.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.