ArticleProceedings of the National Academy of Sciences of the United States of America1990
The spatial distribution of exposed nuclear DNA in normal, cancer, and reverse-transformed cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 48 citations in OpenAlex.
- Chromatin dynamics.Annual review of biophysics · 2010Review
- Contributions of extracellular matrix signaling and tissue architecture to nuclear mechanisms and spatial organization of gene expression control.Biochimica et biophysica acta · 2009Review
- Epigenetic reversion of breast carcinoma phenotype is accompanied by changes in DNA sequestration as measured by AluI restriction enzyme.The American journal of pathology · 2007Article
- Role of tumor invasiveness, the extracellular matrix, and chromatin sequestration in the susceptibility of uveal melanoma to herpes simplex virus type 1.Experimental eye research · 2007Article
- NuMA influences higher order chromatin organization in human mammary epithelium.Molecular biology of the cell · 2007Article
- Transposition of DNase hypersensitive chromatin to the nuclear periphery coincides temporally with nerve growth factor-induced up-regulation of gene expression in PC12 cells.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Robert Feulgen Prize Lecture 1995. New approaches to in situ detection of nucleic acids.Histochemistry and cell biology · 1995Review
- A proposal for a coherent mammalian histone H1 nomenclature correlated with amino acid sequences.Protein science : a publication of the Protein Society · 1994Article
- Fractionation of human H1 subtypes and characterization of a subtype-specific antibody exhibiting non-uniform nuclear staining.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1993Article
- Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus.The Journal of cell biology · 1993Article
- Discrete nuclear domains of poly(A) RNA and their relationship to the functional organization of the nucleus.The Journal of cell biology · 1991Article
- Preferential distribution of active RNA polymerase II molecules in the nuclear periphery.Gene expression · 1991Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The malignant CHO-K1 cell is reverse-transformed by cAMP, regaining the phenotype of a normal fibroblast. During this reaction, much of its DNA re-acquires sensitivity to hydrolysis by DNase I in a way characteristic of the normal fibroblast. Exposed DNA forms a rim about the nucleus in both the normal and reverse-transformed cell but not in the malignant CHO-K1. Reacquisition of the nuclear rim requires an organized cytoskeleton. Sequestered DNA forms families of different degrees of sequestration. In accordance with previous theoretical developments it is proposed that (i) genes specific to a given differentiation state are stored in the nuclear rim, whereas genes specific to other states are sequestered within the nucleus; (ii) only exposed genes are active, and their activity is modulated by regulatory molecules in the fluid medium; (iii) exposure and sequestration are regulated by cytoskeletal and nuclear protein structures; (iv) in at least several types of cancer the regulatory defect lies in the genome exposure process so that the specific DNA sequences and their associated growth regulatory loci have been transferred from the exposed to the sequestered condition with consequent loss of the nuclear rim of exposed DNA. The methodology described should be generally applicable to examining the accessibility state of subsets of DNA during various physiological modulations of cell function.
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