ArticleNucleic acids research1990
Targeted degradation of mRNA in Xenopus oocytes and embryos directed by modified oligonucleotides: studies of An2 and cyclin in embryogenesis.
Article in Nucleic acids research, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 103 citations in OpenAlex.
- Gapmers: Invention and Early History.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Comparative molecular dynamics calculations of duplexation of chemically modified analogs of DNA used for antisense applications.NAR genomics and bioinformatics · 2024Article
- A lncRNA Perspective into (Re)Building the Heart.Frontiers in cell and developmental biology · 2016Review
- Synthetic strategies for studying embryonic development.Chemistry & biology · 2010Review
- Regulation of cyclin B2 expression and cell cycle G2/m transition by menin.The Journal of biological chemistry · 2010Article
- The use of antisense oligonucleotides in Xenopus oocytes.Methods (San Diego, Calif.) · 2010Review
- Transgenic Xenopus laevis embryos can be generated using phiC31 integrase.Nature methods · 2005Article
- Targeted elimination of zygotic messages in Xenopus laevis embryos by modified oligonucleotides possessing terminal cationic linkages.Nucleic acids research · 2000Article
- Effects of RNA secondary structure on cellular antisense activity.Nucleic acids research · 2000Article
- Positively charged oligonucleotides overcome potassium-mediated inhibition of triplex DNA formation.Nucleic acids research · 1996Article
- Oligodeoxynucleoside phosphoramidates (P-NH2): synthesis and thermal stability of duplexes with DNA and RNA targets.Nucleic acids research · 1996Article
- RNase H is responsible for the non-specific inhibition of in vitro translation by 2'-O-alkyl chimeric oligonucleotides: high affinity or selectivity, a dilemma to design antisense oligomers.Nucleic acids research · 1995Article
- Single base discrimination for ribonuclease H-dependent antisense effects within intact human leukaemia cells.Nucleic acids research · 1995Article
- Design and application of antisense oligonucleotides in cell culture, in vivo, and as therapeutic agents.Cellular and molecular neurobiology · 1994Review
- The role of the 5' untranslated region of eukaryotic messenger RNAs in translation and its investigation using antisense technologies.Progress in nucleic acid research and molecular biology · 1994Review
- A more efficient and specific strategy in the ablation of mRNA in Xenopus laevis using mixtures of antisense oligos.Nucleic acids research · 1993Article
- Intracellular disposition and metabolism of fluorescently-labeled unmodified and modified oligonucleotides microinjected into mammalian cells.Nucleic acids research · 1993Article
- Facile preparation of nuclease resistant 3' modified oligodeoxynucleotides.Nucleic acids research · 1993Article
- 3'-modified antisense oligodeoxyribonucleotides complementary to calmodulin mRNA alter behavioral responses in Paramecium.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Polyalkylcyanoacrylate nanoparticles as polymeric carriers for antisense oligonucleotides.Pharmaceutical research · 1992Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have designed antisense oligodeoxyribonucleotides which are both highly resistant to nucleolytic degradation and also serve as substrates for ribonuclease H. Using these compounds we have targeted the specific degradation of several maternal mRNAs present in Xenopus laevis oocytes and early embryos. Several internucleoside linkages at both the 3' and 5' ends of the oligonucleotides were modified as phosphoramidates to provide complete protection against exonucleases, the predominant nucleolytic activity found in both oocytes and embryos. Eight Internal linkages were left unmodified to provide a substrate for RNase H. Degradation of specific embryonic mRNAs was accomplished using subtoxic amounts of the modified oligonucleotides. Specific depletion of An2, a localized mRNA encoding the alpha subunit of the mitochondrial ATPase, produced embryos that gastrulated later than control embryos and arrested in development prior to neurulation. A modified oligonucleotide targeting Xenopus cyclin B1 and cyclin B2 mRNA was also synthesized. Following the injection of one blastomere of a two-cell embryo with the anti-cyclin oligonucleotide, cell division in that half of the embryo was inhibited, demonstrating the in vivo importance of these cyclins in mitosis. The oligonucleotide analogs described here should be useful in studying developmentally significant proteins in Xenopus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.