ArticleThe Journal of physiology2006
Inactivation of nitric oxide by rat cerebellar slices.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Ordered Motions in the Nitric-Oxide Dioxygenase Mechanism of Flavohemoglobin and Assorted Globins with Tightly Coupled Reductases.Advances in experimental medicine and biology · 2023Article
- Modeling NO Biotransport in Brain Using a Space-Fractional Reaction-Diffusion Equation.Frontiers in physiology · 2021Article
- NChemical biology & drug design · 2019Article
- NO as a multimodal transmitter in the brain: discovery and current status.British journal of pharmacology · 2019Review
- From synaptically localized to volume transmission by nitric oxide.The Journal of physiology · 2016Review
- Leptin-dependent neuronal NO signaling in the preoptic hypothalamus facilitates reproduction.The Journal of clinical investigation · 2014Article
- Cellular targets of nitric oxide in the hippocampus.PloS one · 2013Article
- Fluorogenic tagging of protein 3-nitrotyrosine with 4-(aminomethyl)benzene sulfonate in tissues: a useful alternative to Immunohistochemistry for fluorescence microscopy imaging of protein nitration.Free radical biology & medicine · 2012Article
- Role of nitric oxide in cerebellar development and function: focus on granule neurons.Cerebellum (London, England) · 2012Review
- Hemoglobin: a nitric-oxide dioxygenase.Scientifica · 2012Review
- Nitric oxide inactivation mechanisms in the brain: role in bioenergetics and neurodegeneration.International journal of cell biology · 2012Article
- Picomolar nitric oxide signals from central neurons recorded using ultrasensitive detector cells.The Journal of biological chemistry · 2011Article
- Nitric oxide donors enhance the frequency dependence of dopamine release in nucleus accumbens.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2011Article
- Information processing deficits and nitric oxide signalling in the phencyclidine model of schizophrenia.Psychopharmacology · 2010Article
- Transgenic neuronal nitric oxide synthase expression induces axotomy-like changes in adult motoneurons.The Journal of physiology · 2010Article
- Hyperpolarization-activated ion channels as targets for nitric oxide signalling in deep cerebellar nuclei.The European journal of neuroscience · 2010Article
- What is the real physiological NO concentration in vivo?Nitric oxide : biology and chemistry · 2009Review
- Cytochrome P450 oxidoreductase participates in nitric oxide consumption by rat brain.The Biochemical journal · 2009Article
- Assessing the physiological concentration and targets of nitric oxide in brain tissue.The Journal of physiology · 2008Article
- Concepts of neural nitric oxide-mediated transmission.The European journal of neuroscience · 2008Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Nitric oxide (NO) functions as an intercellular messenger throughout the brain. For this role to be performed efficiently, there must be a mechanism for neutralizing NO, but whether an active biological process exists, or whether NO is lost mainly through diffusion is unclear. To investigate this issue, rat cerebellar slices were exposed to constant levels of NO and the cGMP generated within the slice used as an indicator of NO concentrations therein. NO was about 1000-fold less potent in slices (EC50, 1 microM) than in separated cells from the same tissue (EC50, 1.6 nM), consistent with access of NO to the slice interior being greatly hindered by inactivation. Supporting this interpretation, immunohistochemical analysis indicated a marked concentration gradient of cGMP across the thickness of slices exposed to subsaturating NO concentrations, signifying a marked NO gradient. Several known NO-degrading processes, including reaction with lipid peroxyl radicals, erythrocytes and superoxide ions, were eliminated as contributing factors, indicating a novel mechanism. A diffusion-inactivation model was used to estimate the kinetics of NO consumption by the slices. The best fits to experimental data indicated a Michaelis-Menten-type reaction having a Vmax of 1-2 microM s-1 and a Km of around 10 nM. The rates predict that inactivation would impose a very short half-life (<10 ms) on NO in physiological concentrations (up to 10 nM) and that it would play an important role in shaping the NO concentration profiles when it is synthesized by multiple nearby sites.
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Registered trials
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