ArticleThe Journal of cell biology1990
A single human keratin 18 gene is expressed in diverse epithelial cells of transgenic mice.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 100 citations in OpenAlex.
- Article
- Update of the keratin gene family: evolution, tissue-specific expression patterns, and relevance to clinical disorders.Human genomics · 2022Article
- SARS-CoV-2 Infects Human Engineered Heart Tissues and Models COVID-19 Myocarditis.JACC. Basic to translational science · 2021Article
- High-Throughput Screening for Drugs that Modulate Intermediate Filament Proteins.Methods in enzymology · 2016Article
- Precursor cells from Atlantic salmon (Salmo salar) visceral fat holds the plasticity to differentiate into the osteogenic lineage.Biology open · 2015Article
- Identification of a mimotope for circulating anti-cytokeratin 8/18 antibody and its usage for the diagnosis of breast cancer.International journal of oncology · 2013Article
- Serum cytokeratin 18 and cytokine elevations suggest a high prevalence of occupational liver disease in highly exposed elastomer/polymer workers.Journal of occupational and environmental medicine · 2011Article
- Keratin hypersumoylation alters filament dynamics and is a marker for human liver disease and keratin mutation.The Journal of biological chemistry · 2011Article
- Cytoskeletal keratin glycosylation protects epithelial tissue from injury.Nature cell biology · 2010Article
- Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P positive foci transformation into hepatocellular carcinomas.Toxicology and applied pharmacology · 2009Article
- Keratin overexpression levels correlate with the extent of spontaneous pancreatic injury.The American journal of pathology · 2008Article
- Differential sensitivity of mouse epithelial tissues to the polyomavirus middle T oncogene.The American journal of pathology · 2006Article
- Keratin 8 overexpression promotes mouse Mallory body formation.The Journal of cell biology · 2005Article
- Keratin 20 helps maintain intermediate filament organization in intestinal epithelia.Molecular biology of the cell · 2003Article
- Expression of conditional cre recombinase in epithelial tissues of transgenic mice.Genesis (New York, N.Y. : 2000) · 2003Article
- Formation of Mallory body-like inclusions and cell death induced by deregulated expression of keratin 18.Molecular biology of the cell · 2002Article
- Keratin binding to 14-3-3 proteins modulates keratin filaments and hepatocyte mitotic progression.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Mutation of a major keratin phosphorylation site predisposes to hepatotoxic injury in transgenic mice.The Journal of cell biology · 1998Article
- Regulation of keratin and integrin gene expression in cancer and drug resistance.Cytotechnology · 1998Article
- Lessons from keratin 18 knockout mice: formation of novel keratin filaments, secondary loss of keratin 7 and accumulation of liver-specific keratin 8-positive aggregates.The Journal of cell biology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The expression of keratin 18 (K18) is restricted in humans primarily to a variety of single layered or simple epithelia. However, direct introduction of a cloned K18 gene into cultured, somatic cells by DNA transfection has been shown to result in the promiscuous expression of K18 even while the endogenous mouse form of K18 (Endo B) remains silent. To determine if the cloned K18 genomic DNA fragment contains sufficient information to be regulated appropriately when subjected to a normal developmental environment, and to determine if the cloned gene is expressed in diverse epithelia, the K18 gene, including 2.5 kb of 5' flanking sequence and 3.5 kb of 3' flanking sequence, has been introduced into the germ line of mice. Mice from all three resulting K18 transgenic lines express the gene in an appropriate tissue-specific pattern that includes hepatocytes, simple epithelia of the intestinal tract, ductal cells of several glands and epithelial cells of the thymus. No expression of K18 was found in muscle, heart, or in most of the brain even in mice carrying 18 copies of the K18 gene. In most tissues, the level of K18 RNA was directly proportional to copy number and was as efficiently expressed as the endogenous Endo B gene. The K18 protein was identified by both protein blotting methods and indirect immunofluorescence staining. No pathological consequences of overexpression of the K18 gene were observed. The cloned K18 gene appears to contain all cis-acting DNA sequences necessary for appropriate expression. In addition, diverse epithelial cell types are able to express this single human gene.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.