ArticlePlant cell reports2007
Genetic transformation of Gentiana macrophylla with Agrobacterium rhizogenes: growth and production of secoiridoid glucoside gentiopicroside in transformed hairy root cultures.
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 105 citations in OpenAlex.
- Defining Phytochemical Metabolomes of Somatic HybridsMolecules (Basel, Switzerland) · 2025Article
- Anti-Inflammatory Properties of Extract of the Hairy Root of NativeIranian journal of biotechnology · 2023Article
- Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum.Frontiers in plant science · 2022Article
- Enhanced Production of β-Caryophyllene by Farnesyl Diphosphate Precursor-Treated Callus and Hairy Root Cultures ofFrontiers in plant science · 2021Article
- Production of phenolic acids in hairy root cultures of medicinal plantMolecular biology research communications · 2020Article
- Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures ofMolecules (Basel, Switzerland) · 2019Article
- Effect of Different Agrobacterium rhizogenes Strains on Hairy Root Induction and Phenylpropanoid Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum Gaertn).Frontiers in microbiology · 2016Article
- Improved Agrobacterium rhizogenes-mediated hairy root culture system of Withania somnifera (L.) Dunal using sonication and heat treatment.3 Biotech · 2015Article
- Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production.Plant cell reports · 2015Article
- Establishment of hairy root lines and analysis of iridoids and secoiridoids in the medicinal plant Gentiana scabra.Botanical studies · 2014Article
- Production of anthraquinones, phenolic compounds and biological activities from hairy root cultures of Polygonum multiflorum Thunb.Protoplasma · 2014Article
- Efficiency of different Agrobacterium rhizogenes strains on hairy roots induction in Solanum mammosum.World journal of microbiology & biotechnology · 2013Article
- Hairy root cultures of butterfly pea (Clitoria ternatea L.): Agrobacterium × plant factors influencing transformation.World journal of microbiology & biotechnology · 2012Article
- An efficient protocol for genetic transformation of Platycodon grandiflorum with Agrobacterium rhizogenes.Molecular biology reports · 2011Article
- Agrobacterium rhizogenes-mediated transformation of the parasitic plant Phtheirospermum japonicum.PloS one · 2011Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hairy root cultures of Gentiana macrophylla were established by infecting the different explants four Agrobacterium rhizogenes strains namely A(4)GUS, R1000, LBA 9402 and ATCC11325, and hairy root lines were established with A. rhizogenes strain R1000 in 1/2 MS + B(5) medium. Initially, 42 independent hairy root clones were maintained and seven clones belongs to different category were evaluated for growth, morphology, integration and expression of Ri T-DNA genes, and alkaloid contents in dry root samples. On the basis of total root elongation, lateral root density and biomass accumulation on solid media, hairy root clones were separated into three categories. PCR and Southern hybridization analysis revealed both left and right T-DNA integration in the root clones and RT-PCR analysis confirmed the expression of hairy root inducible gene. GUS assay was also performed to confirm the integration of left T-DNA. The accumulation of considerable amounts of the root-specific secoiridoid glucosides gentiopicroside was observed in GM1 (T +/L and T +/R) and the GM2 (T +/L and T -/R DNA) type clones in considerably higher amount whether as two T -/L but T +/R callus-type clones (GM3) accumulated much less or only very negligible amounts of gentiopicroside. Out of four media composition the 1/2 MS + B(5) vitamin media was found most suitable. We found that initial establishment of root cultures largely depends on root:media ratio. Maximum growth rate was recorded in 1:50 root:media ratio. The maximum biomass in terms of fresh weight (33-fold) was achieved in 1/2 MS + B(5) media composition after 35 days in comparison to sixfold increase in control. The biomass increase was most abundant maximum from 15 to 30 days. Influence of A. rhizogenes strains and Ri plasmid of hairy root induction, the possible role of the T(L)-DNA and T(R)-DNA genes on growth pattern of hairy root, initial root inoculum:media ratio and effect of media composition is discussed.
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