ArticleJournal of clinical microbiology2006
Identification of Clostridium species and DNA fingerprinting of Clostridium perfringens by amplified fragment length polymorphism analysis.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Guideline
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- Investigation into the bacterial diversity of sediment samples obtained from Berg River, Western Cape, South Africa.Folia microbiologica · 2021Article
- Comparative Genome Analysis and Spore Heat Resistance Assay Reveal a New Component to Population Structure and Genome Epidemiology WithinFrontiers in microbiology · 2021Article
- Avian PathogenicMicroorganisms · 2020Review
- Pan-Genomic Analysis ofToxins · 2020Article
- Phylogenomic analysis of gastroenteritis-associatedMicrobial genomics · 2019Article
- Systematic Assessment of Nonproteolytic Clostridium botulinum Spores for Heat Resistance.Applied and environmental microbiology · 2016Review
- Historical and current perspectives on Clostridium botulinum diversity.Research in microbiology · 2015Review
- Infant botulism with prolonged faecal excretion of botulinum neurotoxin and Clostridium botulinum for 7 months.Epidemiology and infection · 2014Article
- Genomic and physiological variability within Group II (non-proteolytic) Clostridium botulinum.BMC genomics · 2013Article
- Article
- Comparative genomic hybridization analysis of two predominant Nordic group I (proteolytic) Clostridium botulinum type B clusters.Applied and environmental microbiology · 2009Article
- Long lasting persistence of Bacillus thuringiensis Subsp. israelensis (Bti) in mosquito natural habitats.PloS one · 2008Article
- Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry for identification of Clostridium species isolated from Saudi Arabia.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An amplified fragment length polymorphism (AFLP) method was applied to 129 strains representing 24 different Clostridium species, with special emphasis on pathogenic clostridia of medical or veterinary interest, to assess the potential of AFLP for identification of clostridia. In addition, the ability of the same AFLP protocol to type clostridia at the strain level was assessed by focusing on Clostridium perfringens strains. All strains were typeable by AFLP, so the method seemed to overcome the problem of extracellular DNase production. AFLP differentiated all Clostridium species tested, except for Clostridium ramosum and Clostridium limosum, which clustered together with a 45% similarity level. Other Clostridium species were divided into species-specific clusters or occupied separate positions. Wide genetic diversity was observed among Clostridium botulinum strains, which were divided into seven species-specific clusters. The same AFLP protocol was also suitable for typing C. perfringens at the strain level. A total of 29 different AFLP types were identified for 37 strains of C. perfringens; strains initially originating from the same isolate showed identical fingerprinting patterns and were distinguished from unrelated strains. AFLP proved to be a highly reproducible, easy-to-perform, and relatively fast method which enables high throughput of samples and can serve in the generation of identification libraries. These results indicate that the AFLP method provides a promising tool for the identification and characterization of Clostridium species.
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