ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2006
Regulation of store-operated calcium entry by calcium-independent phospholipase A2 in rat cerebellar astrocytes.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 92 citations in OpenAlex.
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- BTP2, a store-operated calcium channel inhibitor, attenuates morphine antinociceptive tolerance in rats.Frontiers in neuroscience · 2026Article
- SOCE as a regulator of neuronal activity.The Journal of physiology · 2024Review
- The Impact of the Antipsychotic Medication Chlorpromazine on Cytotoxicity through CaNeurotoxicity research · 2022Article
- iPLACells · 2021Article
- Massive efflux of adenosine triphosphate into the extracellular space immediately after experimental traumatic brain injury.Experimental and therapeutic medicine · 2021Article
- Astrocytes respond to a neurotoxic Aβ fragment with state-dependent CaActa neuropathologica communications · 2021Article
- Pluripotent Stem Cell Derived Neurons as In Vitro Models for Studying Autosomal Recessive Parkinson's Disease (ARPD): PLA2G6 and Other Gene Loci.Advances in experimental medicine and biology · 2021Article
- Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide.Frontiers in cellular neuroscience · 2021Article
- Norepinephrine-Induced Calcium Signaling and Store-Operated Calcium Entry in Olfactory Bulb Astrocytes.Frontiers in cellular neuroscience · 2021Article
- Sex-dependent calcium hyperactivity due to lysosomal-related dysfunction in astrocytes from APOE4 versus APOE3 gene targeted replacement mice.Molecular neurodegeneration · 2020Article
- Dopamine-induced calcium signaling in olfactory bulb astrocytes.Scientific reports · 2020Article
- Store-Operated Calcium Channels in Physiological and Pathological States of the Nervous System.Frontiers in cellular neuroscience · 2020Review
- Purinergic Signaling in the Vertebrate Olfactory System.Frontiers in cellular neuroscience · 2019Review
- AMPA Receptor-Mediated CaFrontiers in cellular neuroscience · 2019Article
- Calcium release-activated calcium channels and pain.Cell calcium · 2018Review
- Store-operated calcium entry is essential for glial calcium signalling in CNS white matter.Brain structure & function · 2017Article
- Oxygen and Glucose Deprivation Induces Bergmann Glia Membrane Depolarization and CaFrontiers in cellular neuroscience · 2017Article
- Translationally Controlled Tumor Protein Stimulates Dopamine Release from PC12 Cells via CaInternational journal of molecular sciences · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied store-operated Ca2+ entry (SOCE) in Bergmann glia and granule cell layer astrocytes in acute brain slices of the rat cerebellum, using the Ca2+-sensitive fluorescent dye Fluo-4 and confocal laser scanning microscopy. Astrocytes were identified by their morphology, location, and their Ca2+ response in K+-free solution. Depletion of Ca2+ stores by cyclopiazonic acid (CPA) (20 microM) induced SOCE in both types of astrocyte. A similar Ca2+ influx was elicited by the calmodulin antagonist calmidazolium (CMZ) (1 microM). The SOCE channel blocker 2-aminoethoxy-diphenylborate (2-APB) (100 microM) and the Ca2+ release-activated channel blocker 3,5-bistrifluoromethyl pyrazole derivative (BTP2) (20 microM) suppressed the CPA- and the CMZ-induced Ca2+ influx. Pretreatment of acute slices with the specific Ca2+-independent phospholipase A2 (iPLA2) inhibitor bromoenol lactone (BEL) (25 microM) blocked the CPA- and the CMZ-induced Ca2+ influx. The lysophospholipid products of iPLA2, lysophosphatidylcholine (250 nM) and lysophosphatidylinositol (250 nM), but not lysophosphatidic acid (250 nM), induced a BTP2- and 2-APB-sensitive, but BEL-insensitive, Ca2+ influx. CPA or CMZ enhanced the BEL-sensitive enzymatic activity of iPLA2 in cerebellar astrocyte culture. Inhibition of iPLA2 expression by specific antisense oligodeoxynucleotide of iPLA2 reduced the SOCE and the Ca2+ store refilling in cultured astrocytes. Spontaneous Ca2+ oscillations in astrocytes in situ were reduced after inhibiting SOCE channels or iPLA2 activity. The results suggest that the depletion of Ca2+ stores activates iPLA2 to open Ca2+ channels in the plasma membrane by the formation of lysophospholipids in astrocytes, presumably to refill the stores and allow normal Ca2+ signaling.
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