ArticleNucleic acids research2006
A simple DNA stretching method for fluorescence imaging of single DNA molecules.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- A simple cut and stretch assay to detect antimicrobial resistance genes on bacterial plasmids by single-molecule fluorescence microscopy.Scientific reports · 2022Article
- Thermoplastic nanofluidic devices for identifying abasic sites in single DNA molecules.Lab on a chip · 2021Article
- A micropatterned substrate for on-surface enzymatic labelling of linearized long DNA molecules.Scientific reports · 2019Article
- Sizing femtogram amounts of dsDNA by single-molecule counting.Nucleic acids research · 2016Article
- Article
- Direct optical mapping of transcription factor binding sites on field-stretched λ-DNA in nanofluidic devices.Nucleic acids research · 2014Article
- Novel glass slide preparation system for single DNA molecules analysis.Biotechnology, biotechnological equipment · 2014Article
- Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.Chemical reviews · 2013Review
- Enhancing single molecule imaging in optofluidics and microfluidics.International journal of molecular sciences · 2011Review
- Enzymatic digestion of single DNA molecules anchored on nanogold-modified surfaces.Nanoscale research letters · 2009Article
- See me, feel me: methods to concurrently visualize and manipulate single DNA molecules and associated proteins.Nucleic acids research · 2008Review
- Rapid DNA mapping by fluorescent single molecule detection.Nucleic acids research · 2007Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Stretching or aligning DNA molecules onto a surface by means of molecular combing techniques is one of the critical steps in single DNA molecule analysis. However, many of the current studies have focused on lambda-DNA, or other large DNA molecules. There are very few studies on stretching methodologies for DNA molecules generated via PCR (typically smaller than 20 kb). Here we describe a simple method of stretching DNA molecules up to 18 kb in size on a modified glass surface. The very low background fluorescence allows efficient detection of single fluorescent dye labels incorporated into the stretched DNA molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.