ArticleMolecular and cellular biology1990
Expression of the neurotransmitter-synthesizing enzyme glutamic acid decarboxylase in male germ cells.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 64 citations in OpenAlex.
- Uncovering sperm metabolome to discover biomarkers for bull fertility.BMC genomics · 2019Article
- Expression of multiple glutamate transporter splice variants in the rodent testis.Asian journal of andrology · 2011Article
- Two distinct forms of the 64,000 Mr protein of the cleavage stimulation factor are expressed in mouse male germ cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Structure and alternative promoters of the mouse glutamic acid decarboxylase 67 gene.The Biochemical journal · 1997Article
- A signal located within amino acids 1-27 of GAD65 is required for its targeting to the Golgi complex region.The Journal of cell biology · 1994Article
- Identification of a dominant epitope of glutamic acid decarboxylase (GAD-65) recognized by autoantibodies in stiff-man syndrome.The Journal of experimental medicine · 1993Article
- The synaptic vesicle-associated protein amphiphysin is the 128-kD autoantigen of Stiff-Man syndrome with breast cancer.The Journal of experimental medicine · 1993Article
- Demonstration of GAD-65 as the main immunogenic isoform of glutamate decarboxylase in type 1 diabetes and determination of autoantibodies using a radioligand produced by eukaryotic expression.The Journal of clinical investigation · 1993Article
- Association of GAD-65, but not of GAD-67, with the Golgi complex of transfected Chinese hamster ovary cells mediated by the N-terminal region.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Article
- Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Cloning, characterization, and autoimmune recognition of rat islet glutamic acid decarboxylase in insulin-dependent diabetes mellitus.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Cloning and primary structure of a human islet isoform of glutamic acid decarboxylase from chromosome 10.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- New nucleotide sequence data on the EMBL File Server.Nucleic acids research · 1991Article
- Expression of choline acetyltransferase mRNA in spermatogenic cells results in an accumulation of the enzyme in the postacrosomal region of mature spermatozoa.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- GAD, diabetes, and Stiff-Man syndrome: some progress and more questions.Journal of endocrinological investigationReview
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8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene encoding glutamic acid decarboxylase (GAD), the key enzyme in the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid, is shown to be expressed in the testis of several different species. Nucleotide sequence analysis of a cDNA clone isolated from the human testis confirmed the presence of GAD mRNA in the testis. The major GAD mRNA in the testis was 2.5 kilobases. Smaller amounts of a 3.7-kilobase mRNA with the same size as GAD mRNA in the brain was also detected in the testis. In situ hybridization using a GAD-specific probe revealed GAD mRNA expressing spermatocytes and spermatids located in the middle part of rat seminiferous tubules. Studies on the ontogeny of GAD mRNA expression showed low levels of GAD mRNA in testes of prepubertal rats, with increasing levels as sexual maturation is reached, compatible with GAD mRNA expression in germ cells. In agreement with this, fractionation of cells from the rat seminiferous epithelium followed by Northern (RNA) blot analysis showed the highest levels of GAD mRNA associated with spermatocytes and spermatids. Evidence for the presence of GAD protein in the rat testis was obtained from the demonstration of GAD-like immunoreactivity in seminiferous tubules, predominantly at a position where spermatids and spermatozoa are found. Furthermore, GAD-like immunoreactivity was seen in the midpiece of ejaculated human spermatozoa, the part that is responsible for generating energy for spermatozoan motility.
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