ArticleJournal of cell science2006
Trimerisation is important for the function of clathrin at the mitotic spindle.
Article in Journal of cell science, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- A conserved interaction between the effector Sca4 and host clathrin suggests additional contributions for Sca4 during rickettsial infection.Infection and immunity · 2024Article
- Clathrin mediates membrane fission and budding by constricting membrane pores.Cell discovery · 2024Article
- Clathrin Heavy Chain 1 Plays Essential Roles During Oocyte Meiotic Spindle Formation and Early Embryonic Development in Sheep.Frontiers in cell and developmental biology · 2021Article
- Clathrin's adaptor interaction sites are repurposed to stabilize microtubules during mitosis.The Journal of cell biology · 2020Article
- The Clathrin-dependent Spindle Proteome.Molecular & cellular proteomics : MCP · 2016Article
- Synthesis of the Pitstop family of clathrin inhibitors.Nature protocols · 2014Article
- Coordination of adjacent domains mediates TACC3-ch-TOG-clathrin assembly and mitotic spindle binding.The Journal of cell biology · 2013Article
- Inhibition of clathrin by pitstop 2 activates the spindle assembly checkpoint and induces cell death in dividing HeLa cancer cells.Molecular cancer · 2013Article
- Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG.The Journal of cell biology · 2012Article
- The role of clathrin in mitotic spindle organisation.Journal of cell science · 2012Review
- Functional analysis of interaction sites on the N-terminal domain of clathrin heavy chain.Traffic (Copenhagen, Denmark) · 2012Article
- Endocytosis and signaling: cell logistics shape the eukaryotic cell plan.Physiological reviews · 2012Review
- Novel functions of endocytic player clathrin in mitosis.Cell research · 2011Review
- Recruitment of cellular clathrin to viral factories and disruption of clathrin-dependent trafficking.Traffic (Copenhagen, Denmark) · 2011Article
- Aurora A kinase activity is required for localization of TACC3/ch-TOG/clathrin inter-microtubule bridges.Communicative & integrative biology · 2011Article
- Mitotic moonlighting functions for membrane trafficking proteins.Traffic (Copenhagen, Denmark) · 2011Review
- Clathrin heavy chain gene fusions expressed in human cancers: analysis of cellular functions.Traffic (Copenhagen, Denmark) · 2011Article
- Pulling it together: The mitotic function of TACC3.Bioarchitecture · 2011Article
- A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging.The EMBO journal · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Clathrin is a triskelion consisting of three heavy chains each with an associated light chain. During mitosis, clathrin contributes to kinetochore fibre stability. As the N-terminal domain at the foot of each leg can bind to the mitotic spindle, we proposed previously a ;bridge hypothesis' wherein clathrin acts as a brace between two or three microtubules within a kinetochore fibre to increase fibre stability. Here, we have tested this hypothesis by replacing endogenous clathrin heavy chain in human cells with a panel of clathrin constructs. Mutants designed to abolish trimerisation were unable to rescue the mitotic defects caused by depletion of endogenous clathrin. By contrast, stunted triskelia with contracted legs could partially rescue normal mitosis. These results indicate that the key structural features of clathrin that are necessary for its function in mitosis are a trimeric molecule with a spindle interaction domain at each end, supporting the bridge hypothesis for clathrin function in mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.