ArticleJournal of neurobiology2006
Tissue inhibitor of metalloproteinase-2 (TIMP-2) regulates neuromuscular junction development via a beta1 integrin-mediated mechanism.
Article in Journal of neurobiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Effects of Paraspinal Intramuscular Injection of Atelocollagen in Patients with Chronic Low Back Pain: A Retrospective Observational Study.Journal of clinical medicine · 2024Article
- Bioinformatics and Systems Biology Approach to Identify the Pathogenetic Link between Heart Failure and Sarcopenia.Arquivos brasileiros de cardiologia · 2023Article
- Roles of Matrix Metalloproteinases and Their Natural Inhibitors in Metabolism: Insights into Health and Disease.International journal of molecular sciences · 2023Review
- Breast Cancer Treatment Decreases Serum Levels of TGF-β1, VEGFR2, and TIMP-2 Compared to Healthy Volunteers: Significance for Therapeutic Outcomes?Pathophysiology : the official journal of the International Society for Pathophysiology · 2022Article
- Unravelling the distinct biological functions and potential therapeutic applications of TIMP2 in cancer.Carcinogenesis · 2022Review
- Secreted tissue inhibitor of matrix metalloproteinase restrictsJournal of cell science · 2017Article
- Conditioned media from human palatine tonsil mesenchymal stem cells regulates the interaction between myotubes and fibroblasts by IL-1Ra activity.Journal of cellular and molecular medicine · 2017Article
- Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis.Methods in molecular biology (Clifton, N.J.) · 2017Article
- TIMP-2 modulates cancer cell transcriptional profile and enhances E-cadherin/beta-catenin complex expression in A549 lung cancer cells.Oncotarget · 2013Article
- Tissue inhibitor of metalloproteinases (TIMPs) in heart failure.Heart failure reviews · 2012Review
- A rapid lung de-cellularization protocol supports embryonic stem cell differentiation in vitro and following implantation.Tissue engineering. Part C, Methods · 2012Article
- Comparative assessment of detergent-based protocols for mouse lung de-cellularization and re-cellularization.Tissue engineering. Part C, Methods · 2012Article
- Extracellular matrix and its receptors in Drosophila neural development.Developmental neurobiology · 2011Review
- Endogenous angiogenesis inhibitor blocks tumor growth via direct and indirect effects on tumor microenvironment.The American journal of pathology · 2011Article
- Aberrant repair and fibrosis development in skeletal muscle.Skeletal muscle · 2011Article
- Sexually dimorphic diet-induced insulin resistance in obese tissue inhibitor of metalloproteinase-2 (TIMP-2)-deficient mice.Endocrinology · 2011Article
- Hyperphagia and leptin resistance in tissue inhibitor of metalloproteinase-2 deficient mice.Journal of neuroendocrinology · 2011Article
- The tissue inhibitors of metalloproteinases (TIMPs): an ancient family with structural and functional diversity.Biochimica et biophysica acta · 2010Review
- Tissue inhibitor of metalloproteinase-2 (TIMP-2) regulates myogenesis and beta1 integrin expression in vitro.Experimental cell research · 2008Article
- Potential regulatory relationship between the nested gene DDC8 and its host gene tissue inhibitor of metalloproteinase-2.Physiological genomics · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Extracellular matrix (ECM) molecules play critical roles in muscle function by participating in neuromuscular junction (NMJ) development and the establishment of stable, cytoskeleton-associated adhesions required for muscle contraction. Matrix metalloproteinases (MMPs) are neutral endopeptidases that degrade all ECM components. While the role of MMPs and their inhibitors, the tissue inhibitor of metalloproteinases (TIMPs), has been investigated in many tissues, little is known about their role in muscle development and mature function. TIMP-2 -/- mice display signs of muscle weakness. Here, we report that TIMP-2 is expressed at the NMJ and its expression is greater in fast-twitch (extensor digitorum longus, EDL) than slow-twitch (soleus) muscle. EDL muscle mass is reduced in TIMP-2-/- mice without a concomitant change in fiber diameter or number. The TIMP-2-/- phenotype is not likely due to increased ECM proteolysis because net MMP activity is actually reduced in TIMP-2-/- muscle. Most strikingly, TIMP-2 colocalizes with beta1 integrin at costameres in the wild-type EDL and beta1 integrin expression is significantly reduced in TIMP-2-/- EDL. We propose that reduced beta1 integrin in fast-twitch muscle may be associated with destabilized ECM-cytoskeletal interactions required for muscle contraction in TIMP-2-/- muscle; thus, explaining the muscle weakness. Given that fast-twitch fibers are lost in muscular dystrophies and age-related sarcopenia, if TIMP-2 regulates mechanotransduction in an MMP-independent manner it opens new potential therapeutic avenues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.