ArticleInfection and immunity2006
Role of lgtC in resistance of nontypeable Haemophilus influenzae strain R2866 to human serum.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- A cell-permeable probe for the labelling of a bacterial glycosyltransferase and virulence factor.RSC chemical biology · 2024Article
- The DmsABC Sulfoxide Reductase Supports Virulence in Non-typeableFrontiers in microbiology · 2021Article
- Underlying Glycans Determine the Ability of Sialylated Lipooligosaccharide To Protect Nontypeable Haemophilus influenzae from Serum IgM and Complement.Infection and immunity · 2019Article
- Uptake of Sialic Acid by Nontypeable Haemophilus influenzae Increases Complement Resistance through Decreasing IgM-Dependent Complement Activation.Infection and immunity · 2019Article
- Identification of non-substrate-like glycosyltransferase inhibitors from library screening: pitfalls & hits.MedChemComm · 2018Article
- The modA10 phasevarion of nontypeable Haemophilus influenzae R2866 regulates multiple virulence-associated traits.Microbial pathogenesis · 2016Article
- A Novel, Molybdenum-Containing Methionine Sulfoxide Reductase Supports Survival ofFrontiers in microbiology · 2016Article
- Maturation of molybdoenzymes and its influence on the pathogenesis of non-typeable Haemophilus influenzae.Frontiers in microbiology · 2015Article
- Peroxiredoxin-glutaredoxin and catalase promote resistance of nontypeable Haemophilus influenzae 86-028NP to oxidants and survival within neutrophil extracellular traps.Infection and immunity · 2015Article
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- Evasion of killing by human antibody and complement through multiple variations in the surface oligosaccharide of Haemophilus influenzae.Molecular microbiology · 2013Article
- Adhesin genes and serum resistance in Haemophilus influenzae type f isolates.Journal of medical microbiology · 2013Article
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- Infant rat infection modifies phenotypic properties of an invasive nontypeable Haemophilus influenzae.Microbes and infection · 2012Article
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- Phosphorylcholine allows for evasion of bactericidal antibody by Haemophilus influenzae.PLoS pathogens · 2012Article
- RbsB (NTHI_0632) mediates quorum signal uptake in nontypeable Haemophilus influenzae strain 86-028NP.Molecular microbiology · 2011Article
- Molecular basis of increased serum resistance among pulmonary isolates of non-typeable Haemophilus influenzae.PLoS pathogens · 2011Article
- Binding of complement regulators to invasive nontypeable Haemophilus influenzae isolates is not increased compared to nasopharyngeal isolates, but serum resistance is linked to disease severity.Journal of clinical microbiology · 2010Article
Corrections and comments
- Erratum issuedBonthius, Paul J [corrected to Bonthuis, Paul J]
Authors and funding
13 authors at 5 institutions in 1 country.
Funding
Abstract
We are investigating a nontypeable Haemophilus influenzae (NTHI) strain, R2866, isolated from a child with meningitis. R2866 is unusually resistant to killing by normal human serum. The serum 50% inhibitory concentration (IC50) for this strain is 18%, approaching that of encapsulated H. influenzae. R3392 is a derivative of R2866 that was found to have increased sensitivity to human serum (IC50, 1.5%). Analysis of tetrameric repeat regions within lipooligosaccharide (LOS) biosynthetic genes in both strains indicated that the glycosyltransferase gene lgtC was out of frame ("off") in most colonies of R3392 but in frame with its start codon ("on") in most colonies of the parent. We sought antigenic and biochemical evidence for modification of the LOS structure. In a whole-cell enzyme-linked immunosorbent assay, strain R3392 displayed reduced binding of the Galalpha1,4Gal-specific monoclonal antibody 4C4. Mass spectrometry analysis of LOS from strain R2866 indicated that the primary oligosaccharide glycoform contained four heptose and four hexose residues, while that of R3392 contained four heptose and three hexose residues. We conclude that the R2866 lgtC gene encodes a galactosyltransferase involved in synthesis of the 4C4 epitope, as in other strains, and that expression of lgtC is associated with the high-level serum resistance that has been observed for this strain. This is the first description of the genetic basis of high-level serum resistance in NTHI, as well as the first description of LOS composition in an NTHI strain for which the complete genome sequence has been determined.
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