ArticleAntimicrobial agents and chemotherapy2006
A small subpopulation of blastospores in candida albicans biofilms exhibit resistance to amphotericin B associated with differential regulation of ergosterol and beta-1,6-glucan pathway genes.
Article in Antimicrobial agents and chemotherapy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 102 citations in OpenAlex.
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- Label-free sub-micrometer 3D imaging of ciprofloxacin in native-state biofilms with cryo-time-of-flight secondary ion mass spectrometry.Analytical and bioanalytical chemistry · 2023Article
- Eradication of Candida albicans persister cell biofilm by the membranotropic peptide gH625.Scientific reports · 2020Article
- Antifungal and anti-biofilm activity of the first cryptic antimicrobial peptide from an archaeal protein against Candida spp. clinical isolates.Scientific reports · 2018Article
- Fungal persister cells: The basis for recalcitrant infections?PLoS pathogens · 2018Review
- Echinocandin Treatment of Candida albicans Biofilms Enhances Neutrophil Extracellular Trap Formation.Antimicrobial agents and chemotherapy · 2018Article
- Development and regulation of single- and multi-species Candida albicans biofilms.Nature reviews. Microbiology · 2018Review
- Liposomal and Deoxycholate Amphotericin B Formulations: Effectiveness against Biofilm Infections of Candida spp.Pathogens (Basel, Switzerland) · 2017Article
- Assessment and Optimizations of Candida albicansAntimicrobial agents and chemotherapy · 2017Article
- Candida glabrata Biofilms: How Far Have We Come?Journal of fungi (Basel, Switzerland) · 2017Review
- Candida Species Biofilms' Antifungal Resistance.Journal of fungi (Basel, Switzerland) · 2017Review
- Persistence and drug tolerance in pathogenic yeast.Current genetics · 2017Review
- Antifungal Therapy: New Advances in the Understanding and Treatment of Mycosis.Frontiers in microbiology · 2017Review
- A common mechanism involving the TORC1 pathway can lead to amphotericin B-persistence in biofilm and planktonic Saccharomyces cerevisiae populations.Scientific reports · 2016Article
- Flow Cytometry-Based Method To Detect Persisters in Candida albicans.Antimicrobial agents and chemotherapy · 2015Article
- Fungal Biofilms: In Vivo Models for Discovery of Anti-Biofilm Drugs.Microbiology spectrum · 2015Article
- Antifungal activity of amphotericin B and voriconazole against the biofilms and biofilm-dispersed cells of Candida albicans employing a newly developed in vitro pharmacokinetic model.Annals of clinical microbiology and antimicrobials · 2015Article
- Human serum potentiates the expression of genes associated with antifungal drug resistance in C. albicans biofilms on central venous catheters.Mycopathologia · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The resistance of Candida albicans biofilms to a broad spectrum of antimicrobial agents has been well documented. Biofilms are known to be heterogeneous, consisting of microenvironments that may induce formation of resistant subpopulations. In this study we characterized one such subpopulation. C. albicans biofilms were cultured in a tubular flow cell (TF) for 36 h. The relatively large shear forces imposed by draining the TF removed most of the biofilm, which consisted of a tangled mass of filamentous forms with associated clusters of yeast forms. This portion of the biofilm exhibited the classic architecture and morphological heterogeneity of a C. albicans biofilm and was only slightly more resistant than either exponential- or stationary-phase planktonic cells. A submonolayer fraction of blastospores that remained on the substratum was resistant to 10 times the amphotericin B dose that eliminated the activity of the planktonic populations. A comparison between planktonic and biofilm populations of transcript abundance for genes coding for enzymes in the ergosterol (ERG1, -3, -5, -6, -9, -11, and -25) and beta-1,6-glucan (SKN and KRE1, -5, -6, and -9) pathways was performed by quantitative RT-PCR. The results indicate a possible association between the high level of resistance exhibited by the blastospore subpopulation and differential regulation of ERG1, ERG25, SKN1, and KRE1. We hypothesize that the resistance originates from a synergistic effect involving changes in both the cell membrane and the cell wall.
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