ArticleMolecular and cellular biology2006
Transcriptional regulation is affected by subnuclear targeting of reporter plasmids to PML nuclear bodies.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
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- Role of ND10 nuclear bodies in the chromatin repression of HSV-1.Virology journal · 2016Review
- The Role of HDACs as Leukemia Therapy Targets using HDI.International journal of hematology-oncology and stem cell research · 2015Article
- Myosin VI regulates gene pairing and transcriptional pause release in T cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Death domain-associated protein DAXX promotes ovarian cancer development and chemoresistance.The Journal of biological chemistry · 2013Article
- Identifying gene locus associations with promyelocytic leukemia nuclear bodies using immuno-TRAP.The Journal of cell biology · 2013Article
- Physical and functional interaction between PML and TBX2 in the establishment of cellular senescence.The EMBO journal · 2012Article
- Epstein - Barr virus Latent Membrane Protein 1 suppresses reporter activity through modulation of promyelocytic leukemia protein-nuclear bodies.Virology journal · 2011Article
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- Sorting the nuclear proteome.Bioinformatics (Oxford, England) · 2011Article
- PML isoforms I and II participate in PML-dependent restriction of HSV-1 replication.Journal of cell science · 2011Article
- Role of herpes simplex virus ICP0 in the transactivation of genes introduced by infection or transfection: a reappraisal.Journal of virology · 2010Article
- Multipotent stromal cells are activated to reduce apoptosis in part by upregulation and secretion of stanniocalcin-1.Stem cells (Dayton, Ohio) · 2009Article
- Induction of cellular stress overcomes the requirement of herpes simplex virus type 1 for immediate-early protein ICP0 and reactivates expression from quiescent viral genomes.Journal of virology · 2008Article
- Signal-dependent regulation of transcription by histone deacetylase 7 involves recruitment to promyelocytic leukemia protein nuclear bodies.Molecular biology of the cell · 2008Article
- Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair.Molecular and cellular biology · 2008Article
- Hexamethylene bisacetamide leads to reduced helper virus-free HSV-1 amplicon expression titers via suppression of ICP0.The journal of gene medicine · 2008Article
- Induction of promyelocytic leukemia (PML) oncogenic domains (PODs) by papillomavirus.Virology · 2007Article
- Subcellular distribution of human RDM1 protein isoforms and their nucleolar accumulation in response to heat shock and proteotoxic stress.Nucleic acids research · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whereas the PML protein has been reported to have both transcriptional coactivator and corepressor potential, the contribution of the PML nuclear body (PML NB) itself to transcriptional regulation is not well understood. Here we demonstrate that plasmid DNA artificially tethered to PML or the PML NB-targeting domain of Sp100 is preferentially localized to PML NBs. Using the tethering technique, we targeted a simian virus 40 promoter-driven luciferase reporter plasmid to PML NBs, resulting in the repression of the transgene transcriptional activity. Conversely, the tethering of a cytomegalovirus promoter-containing reporter plasmid resulted in activation. Targeting a minimal eukaryotic promoter did not affect its activity. The expression of targeted promoters could be modulated by altering the cellular concentration of PML NB components, including Sp100 and isoforms of the PML protein. Finally, we demonstrate that ICP0, the promiscuous herpes simplex virus transactivator, increases the level of transcriptional activation of plasmid DNA tethered to the PML NB. We conclude that when PML NB components are artificially tethered to reporter plasmids, the PML NB contributes to the regulation of the tethered DNA in a promoter-dependent manner. Our findings demonstrate that transient transcription assays are sensitive to the subnuclear localization of the transgene plasmid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.