ArticleJournal of biomolecular NMR2006
Magic-angle spinning solid-state NMR of a 144 kDa membrane protein complex: E. coli cytochrome bo3 oxidase.
Article in Journal of biomolecular NMR, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed.
- Improving cell-free metabolism through direct integration of artificial respiratory chains.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Protein-Rich Rafts in Hybrid Polymer/Lipid Giant Unilamellar Vesicles.Biomacromolecules · 2024Article
- Cellular production of a de novo membrane cytochrome.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Experimental platform for the functional investigation of membrane proteins in giant unilamellar vesicles.Soft matter · 2022Article
- En route to dynamic life processes by SNARE-mediated fusion of polymer and hybrid membranes.Nature communications · 2021Article
- Modulation of Transmembrane Domain Interactions in Neu Receptor Tyrosine Kinase by Membrane Fluidity and Cholesterol.The Journal of membrane biology · 2019Article
- TScientific reports · 2019Article
- Scavenging of superoxide by a membrane-bound superoxide oxidase.Nature chemical biology · 2018Article
- The lateral distance between a proton pump and ATP synthase determines the ATP-synthesis rate.Scientific reports · 2017Article
- Multidimensional Solid-State Nuclear Magnetic Resonance of a Functional Multiprotein Chemoreceptor Array.Biochemistry · 2016Article
- Lipid-mediated Protein-protein Interactions Modulate Respiration-driven ATP Synthesis.Scientific reports · 2016Article
- Lipid bilayer-bound conformation of an integral membrane beta barrel protein by multidimensional MAS NMR.Journal of biomolecular NMR · 2015Article
- Membrane attachment and structure models of lipid storage droplet protein 1.Biochimica et biophysica acta · 2014Article
- VITAL NMR: using chemical shift derived secondary structure information for a limited set of amino acids to assess homology model accuracy.Journal of biomolecular NMR · 2012Article
- A rapid and robust method for selective isotope labeling of proteins.Methods (San Diego, Calif.) · 2011Article
- Chemical shift correlation at high MAS frequencies employing low-power symmetry-based mixing schemes.Journal of biomolecular NMR · 2011Article
- A proton spin diffusion based solid-state NMR approach for structural studies on aligned samples.The journal of physical chemistry. B · 2011Article
- Atomic view of calcium-induced clustering of phosphatidylserine in mixed lipid bilayers.Biochemistry · 2011Article
- Lipid-protein correlations in nanoscale phospholipid bilayers determined by solid-state nuclear magnetic resonance.Biochemistry · 2010Article
- Automated protein resonance assignments of magic angle spinning solid-state NMR spectra of β1 immunoglobulin binding domain of protein G (GB1).Journal of biomolecular NMR · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Recent progress in magic-angle spinning (MAS) solid-state NMR (SSNMR) has enabled multidimensional studies of large, macroscopically unoriented membrane proteins with associated lipids, without the requirement of solubility that limits other structural techniques. Here we present initial sample preparation and SSNMR studies of a 144 kDa integral membrane protein, E. coli cytochrome bo(3) oxidase. The optimized protocol for expression and purification yields approximately 5 mg of the enzymatically active, uniformly (13)C,(15)N-enriched membrane protein complex from each liter of growth medium. The preparation retains endogenous lipids and yields spectra of high sensitivity and resolution, consistent with a folded, homogenous protein. Line widths of isolated signals are less than 0.5 ppm, with a large number of individual resonances resolved in the 2D and 3D spectra. The (13)C chemical shifts, assigned by amino acid type, are consistent with the secondary structure previously observed by diffraction methods. Although the structure is predominantly helical, the percentage of non-helical signals varies among residue types; these percentages agree well between the NMR and diffraction data. Samples show minimal evidence of degradation after several weeks of NMR data acquisition. Use of a triple resonance scroll resonator probe further improves sample stability and enables higher power decoupling, higher duty cycles and more advanced 3D experiments to be performed. These initial results in cytochrome bo(3) oxidase demonstrate that multidimensional MAS SSNMR techniques have sufficient sensitivity and resolution to interrogate selected parts of a very large uniformly (13)C,(15)N-labeled membrane protein.
Indexed as
Identifiers
16964530What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.