ArticleEukaryotic cell2006
A 60-kilodalton protein component of the counting factor complex regulates group size in Dictyostelium discoideum.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 19 citations in OpenAlex.
- Structure-Function Relationship Study of a Secretory Amoebic Phosphatase: A Computational-Experimental Approach.International journal of molecular sciences · 2021Article
- Cell dispersal by localized degradation of a chemoattractant.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- DPF is a cell-density sensing factor, with cell-autonomous and non-autonomous functions during Dictyostelium growth and development.BMC biology · 2019Article
- The Use of Diffusion Calculations and Monte Carlo Simulations to Understand the Behavior of Cells inComputational and structural biotechnology journal · 2019Review
- Extracellular signaling in Dictyostelium.The International journal of developmental biology · 2019Review
- Loss of FrmB results in increased size of developmental structures during the multicellular development of Dictyostelium cells.Journal of microbiology (Seoul, Korea) · 2017Article
- Different CHD chromatin remodelers are required for expression of distinct gene sets and specific stages during development of Dictyostelium discoideum.Development (Cambridge, England) · 2013Article
- Assets of the non-pathogenic microorganismF1000Research · 2013Review
- Regulation of aggregate size and pattern by adenosine and caffeine in cellular slime molds.BMC developmental biology · 2012Article
- Cell density sensing and size determination.Development, growth & differentiation · 2011Review
- A cell number counting factor alters cell metabolism.Communicative & integrative biology · 2009Article
- Dictyostelium cells bind a secreted autocrine factor that represses cell proliferation.BMC biochemistry · 2009Article
- A protein with similarity to PTEN regulates aggregation territory size by decreasing cyclic AMP pulse size during Dictyostelium discoideum development.Eukaryotic cell · 2008Article
- Combining experiments and modelling to understand size regulation in Dictyostelium discoideum.Journal of the Royal Society, Interface · 2008Review
- The secreted Dictyostelium protein CfaD is a chalone.Journal of cell science · 2008Article
- CnrN regulates Dictyostelium group size using a counting factor-independent mechanism.Communicative & integrative biology · 2008Article
- A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium.Eukaryotic cell · 2007Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Much remains to be understood about how a group of cells or a tissue senses and regulates its size. Dictyostelium discoideum cells sense and regulate the size of groups and fruiting bodies using a secreted 450-kDa complex of proteins called counting factor (CF). Low levels of CF result in large groups, and high levels of CF result in small groups. We previously found three components of CF (D. A. Brock and R. H. Gomer, Genes Dev. 13:1960-1969, 1999; D. A. Brock, R. D. Hatton, D.-V. Giurgiutiu, B. Scott, R. Ammann, and R. H. Gomer, Development 129:3657-3668, 2002; and D. A. Brock, R. D. Hatton, D.-V. Giurgiutiu, B. Scott, W. Jang, R. Ammann, and R. H. Gomer, Eukaryot. Cell 2:788-797, 2003). We describe here a fourth component, CF60. CF60 has similarity to acid phosphatases, although it has very little, if any, acid phosphatase activity. CF60 is secreted by starving cells and is lost from the 450-kDa CF when a different CF component, CF50, is absent. Although we were unable to obtain cells lacking CF60, decreasing CF60 levels by antisense resulted in large groups, and overexpressing CF60 resulted in small groups. When added to wild-type cells, conditioned starvation medium from CF60 overexpressor cells as well as recombinant CF60 caused the formation of small groups. The ability of recombinant CF60 to decrease group size did not require the presence of the CF component CF45-1 or countin but did require the presence of CF50. Recombinant CF60 does not have acid phosphatase activity, indicating that the CF60 bioactivity is not due to a phosphatase activity. Together, the data suggest that CF60 is a component of CF, and thus this secreted signal has four different protein components.
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