ArticleJournal of bacteriology2006
Cooperative and critical roles for both G domains in the GTPase activity and cellular function of ribosome-associated Escherichia coli EngA.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- The Role of the Oral Microbiome in the Development and Treatment of Periodontal Disease: Functional Meta-Omics Evidence.Current microbiology · 2026Review
- Characterizing a novel CMK-EngA fusion protein fromBiochemistry and biophysics reports · 2023Article
- Competitive advantage of oxygen-tolerant bioanodes ofBiofilm · 2021Article
- The Photosystem II Repair Cycle Requires FtsH Turnover through the EngA GTPase.Plant physiology · 2018Article
- YphC and YsxC GTPases assist the maturation of the central protuberance, GTPase associated region and functional core of the 50S ribosomal subunit.Nucleic acids research · 2016Article
- Heterologous Expression of Der Homologs in an Escherichia coli der Mutant and Their Functional Complementation.Journal of bacteriology · 2016Article
- The large ribosomal subunit protein L9 enables the growth of EF-P deficient cells and enhances small subunit maturation.PloS one · 2015Article
- Structural insights into the function of a unique tandem GTPase EngA in bacterial ribosome assembly.Nucleic acids research · 2014Article
- Article
- A genome-scale metabolic flux model of Escherichia coli K-12 derived from the EcoCyc database.BMC systems biology · 2014Article
- Phenotypic investigations of the depletion of EngA in Escherichia coli are consistent with a role in ribosome biogenesis.FEMS microbiology letters · 2014Article
- DER containing two consecutive GTP-binding domains plays an essential role in chloroplast ribosomal RNA processing and ribosome biogenesis in higher plants.Journal of experimental botany · 2014Article
- Evidence for lateral gene transfer (LGT) in the evolution of eubacteria-derived small GTPases in plant organelles.Frontiers in plant science · 2014Article
- A high-throughput screen of the GTPase activity of Escherichia coli EngA to find an inhibitor of bacterial ribosome biogenesis.Journal of biomolecular screening · 2013Article
- Crippling the essential GTPase Der causes dependence on ribosomal protein L9.Journal of bacteriology · 2013Article
- Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation.Proteome science · 2013Article
- Potassium acts as a GTPase-activating element on each nucleotide-binding domain of the essential Bacillus subtilis EngA.PloS one · 2012Article
- Extended C-terminus and length of the linker connecting the G-domains are species-specific variations in the EngA family of GTPases.FEBS open bio · 2012Article
- Article
- The universally conserved prokaryotic GTPases.Microbiology and molecular biology reviews : MMBR · 2011Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To probe the cellular phenotype and biochemical function associated with the G domains of Escherichia coli EngA (YfgK, Der), mutations were created in the phosphate binding loop of each. Neither an S16A nor an S217A variant of G domain 1 or 2, respectively, was able to support growth of an engA conditional null. Polysome profiles of EngA-depleted cells were significantly altered, and His(6)-EngA was found to cofractionate with the 50S ribosomal subunit. The variants were unable to complement the abnormal polysome profile and were furthermore significantly impacted with respect to in vitro GTPase activity. Together, these observations suggest that the G domains have a cooperative function in ribosome stability and/or biogenesis.
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