ArticleJournal of bacteriology2007
C-terminal WxL domain mediates cell wall binding in Enterococcus faecalis and other gram-positive bacteria.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 110 citations in OpenAlex.
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- A conserved sRNA regulates mucin adhesion and gut colonization across the Enterococcaceae.bioRxiv : the preprint server for biology · 2025Article
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- Antigen surface display in two novel whole genome sequenced food grade strains, Lactiplantibacillus pentosus KW1 and KW2.Microbial cell factories · 2024Article
- Bioinformatic analysis of WxL domain proteins.Saudi journal of biological sciences · 2023Article
- Adaptation of the gut pathobiont Enterococcus faecalis to deoxycholate and taurocholate bile acids.Scientific reports · 2022Article
- Variability of Genetic Characters Associated with Probiotic Functions inMicroorganisms · 2022Article
- Identification of WxL and S-Layer Proteins fromInternational journal of molecular sciences · 2022Article
- Bacterium-Like Particles Displaying the Rift Valley Fever Virus Gn Head Protein Induces Efficacious Immune Responses in Immunized Mice.Frontiers in microbiology · 2022Article
- Microdiversity ofEmerging microbes & infections · 2021Article
- Genomic insights into an andean multiresistant soil actinobacterium of biotechnological interest.World journal of microbiology & biotechnology · 2021Article
- Transcriptomics Reveal the Survival Strategies of Enterococcus mundtii in the Gut of Spodoptera littoralis.Journal of chemical ecology · 2021Article
- Development and Characterization of High-ThroughputFrontiers in cellular and infection microbiology · 2021Article
- Engineering Lactococcus lactis as a multi-stress tolerant biosynthetic chassis by deleting the prophage-related fragment.Microbial cell factories · 2020Article
- Strategies to display heterologous proteins on the cell surface of lactic acid bacteria using as anchor the C-terminal domain of Lactobacillus acidophilus SlpA.World journal of microbiology & biotechnology · 2020Article
- A SH3_5 Cell Anchoring Domain for Non-recombinant Surface Display on Lactic Acid Bacteria.Frontiers in bioengineering and biotechnology · 2020Article
- ProbioticThe EPMA journal · 2019Article
- Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analysis of the genome sequence of Enterococcus faecalis clinical isolate V583 revealed novel genes encoding surface proteins. Twenty-seven of these proteins, annotated as having unknown functions, possess a putative N-terminal signal peptide and a conserved C-terminal region characterized by a novel conserved domain designated WxL. Proteins having similar characteristics were also detected in other low-G+C-content gram-positive bacteria. We hypothesized that the WxL region might be a determinant of bacterial cell location. This hypothesis was tested by generating protein fusions between the C-terminal regions of two WxL proteins in E. faecalis and a nuclease reporter protein. We demonstrated that the C-terminal regions of both proteins conferred a cell surface localization to the reporter fusions in E. faecalis. This localization was eliminated by introducing specific deletions into the domains. Interestingly, exogenously added protein fusions displayed binding to whole cells of various gram-positive bacteria. We also showed that the peptidoglycan was a binding ligand for WxL domain attachment to the cell surface and that neither proteins nor carbohydrates were necessary for binding. Based on our findings, we propose that the WxL region is a novel cell wall binding domain in E. faecalis and other gram-positive bacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.