ArticleBiophysical journal2006
DNA as a metrology standard for length and force measurements with optical tweezers.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed.
- Single-molecule measurements of bacteriophage lambda DNA packaging using purified terminase motor proteins and E. coli integration host factor.Scientific reports · 2025Article
- Methods for Studying Motor-Driven Viral DNA Packaging in Bacteriophages phi29, Lambda, and T4 via Single DNA Molecule Manipulation and Rapid Solution Exchange.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Modeling multiple duplex DNA attachments in a force-extension experiment.Biophysical reports · 2022Article
- Determining Trap Compliances, Microsphere Size Variations, and Response Linearities in Single DNA Molecule Elasticity Measurements with Optical Tweezers.Frontiers in molecular biosciences · 2021Article
- Function of a viral genome packaging motor from bacteriophage T4 is insensitive to DNA sequence.Nucleic acids research · 2020Article
- Functional Dissection of a Viral DNA Packaging Machine's Walker B Motif.Journal of molecular biology · 2019Article
- Nucleotide-dependent DNA gripping and an end-clamp mechanism regulate the bacteriophage T4 viral packaging motor.Nature communications · 2018Article
- Walker-A Motif Acts to Coordinate ATP Hydrolysis with Motor Output in Viral DNA Packaging.Journal of molecular biology · 2016Article
- A ruthenium dimer complex with a flexible linker slowly threads between DNA bases in two distinct steps.Nucleic acids research · 2015Article
- Continuous allosteric regulation of a viral packaging motor by a sensor that detects the density and conformation of packaged DNA.Biophysical journal · 2015Article
- Repulsive DNA-DNA interactions accelerate viral DNA packaging in phage Phi29.Physical review letters · 2014Article
- Evidence for an electrostatic mechanism of force generation by the bacteriophage T4 DNA packaging motor.Nature communications · 2014Article
- Nonequilibrium dynamics and ultraslow relaxation of confined DNA during viral packaging.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Accurate measurement of force and displacement with optical tweezers using DNA molecules as metrology standards.Applied physics letters · 2014Article
- Single-molecule studies of viral DNA packaging.Advances in experimental medicine and biology · 2012Review
- Precision steering of an optical trap by electro-optic deflection.Optics letters · 2008Article
- Portal motor velocity and internal force resisting viral DNA packaging in bacteriophage phi29.Biophysical journal · 2008Article
- Elasticity of short DNA molecules: theory and experiment for contour lengths of 0.6-7 microm.Biophysical journal · 2007Article
- Measurements of single DNA molecule packaging dynamics in bacteriophage lambda reveal high forces, high motor processivity, and capsid transformations.Journal of molecular biology · 2007Article
- Single phage T4 DNA packaging motors exhibit large force generation, high velocity, and dynamic variability.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Optical tweezers have broad applications in studies of structures and processes in molecular and cellular biophysics. Use of optical tweezers for quantitative molecular-scale measurement requires careful calibration in physical units. Here we show that DNA molecules may be used as metrology standards for force and length measurements. Analysis of DNA molecules of two specific lengths allows simultaneous determination of all essential measurement parameters. We validate this biological-calibration method experimentally and with simulated data, and show that precisions in determining length scale factor ( approximately 0.2%), length offset ( approximately 0.03%), force scale factor ( approximately 2%), and compliance of the traps ( approximately 3%) are limited only by current measurement variation, much of which arises from polydispersity of the microspheres ( approximately 2%). We find this procedure to be simpler and more convenient than previous methods, and suggest that it provides an easily replicated standard that can insure uniformity of measurements made in different laboratories.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.