ArticleAnalytical biochemistry2006
Identifying decomposition products in extracts of cellular metabolites.
Article in Analytical biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Comparative Metabolomics ofJournal of microbiology and biotechnology · 2025Article
- Hot isopropanol quenching procedure for automated microtiter plate scaleMicrobial cell factories · 2022Article
- Improved Sample Preparation for Untargeted Metabolomics Profiling of Escherichia coli.Microbiology spectrum · 2021Article
- Influence of Extraction Solvent on Nontargeted Metabolomics Analysis of Enrichment Reactor Cultures Performing Enhanced Biological Phosphorus Removal (EBPR).Metabolites · 2021Article
- Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Identifying Biomarkers of Wharton's Jelly Mesenchymal Stromal Cells Using a Dynamic Metabolic Model: The Cell Passage Effect.Metabolites · 2018Article
- Extraction and Quantitation of Nicotinamide Adenine Dinucleotide Redox Cofactors.Antioxidants & redox signaling · 2018Article
- A Dynamic Metabolic Flux Analysis of Myeloid-Derived Suppressor Cells Confirms Immunosuppression-Related Metabolic Plasticity.Scientific reports · 2017Article
- Metabolite Measurement: Pitfalls to Avoid and Practices to Follow.Annual review of biochemistry · 2017Review
- Metabolite labelling reveals hierarchies in Clostridium acetobutylicum that selectively channel carbons from sugar mixtures towards biofuel precursors.Microbial biotechnology · 2017Article
- Applications of NMR spectroscopy to systems biochemistry.Progress in nuclear magnetic resonance spectroscopy · 2016Review
- Impact of storage conditions on metabolite profiles of sputum samples from persons with cystic fibrosis.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2015Article
- Hierarchy in pentose sugar metabolism in Clostridium acetobutylicum.Applied and environmental microbiology · 2015Article
- Biochemical and structural studies of conserved Maf proteins revealed nucleotide pyrophosphatases with a preference for modified nucleotides.Chemistry & biology · 2013Article
- Commitment to glycolysis sustains survival of NO-producing inflammatory dendritic cells.Blood · 2012Article
- Immunosuppressive activity enhances central carbon metabolism and bioenergetics in myeloid-derived suppressor cells in vitro models.BMC cell biology · 2012Article
- Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation.PloS one · 2012Article
- Metabolome remodeling during the acidogenic-solventogenic transition in Clostridium acetobutylicum.Applied and environmental microbiology · 2011Article
- Reducing time and increasing sensitivity in sample preparation for adherent mammalian cell metabolomics.Analytical chemistry · 2011Article
- Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli.Nature chemical biology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Most methods of analyzing intracellular metabolites require extraction of metabolites from the cells. A concern in these methods is underestimation of metabolite levels due to incomplete extraction. In comparing extraction methods, then, it would seem that the best method for extracting a particular metabolite is the one that gives the largest yield. In extracting Escherichia coli with different methanol:water mixtures, we observed that >or=50% water gave an increased yield of nucleosides and bases compared with <or=20% water, as determined by liquid chromatography-tandem mass spectrometry analysis of the resulting extracts. Spiking of the extracts with isotope-labeled nucleotides revealed, however, that the high yield of nucleosides and bases occurred due to decomposition of nucleotides in the water-rich condition, not due to good extraction. Spiking combined with isotope labeling provides a general approach to detecting decomposition products in extracts of cellular metabolites. For extraction of E. coli with methanol:water, cold temperature and a high methanol fraction minimize artifacts due to metabolite decomposition.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.