ArticleThe Journal of cell biology1990
Regulated expression of vimentin cDNA in cells in the presence and absence of a preexisting vimentin filament network.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 143 citations in OpenAlex.
- Type III intermediate filaments as novel CoAlation targets.Redox report : communications in free radical research · 2026Article
- Comprehensive Characterization of Bioactive and Undesirable Compounds in Mezcal-Derived Vinasse for Potential Circular Applications.Foods (Basel, Switzerland) · 2026Article
- Intermediate filament dysregulation in astrocytes in the human disease model ofMolecular biology of the cell · 2023Article
- Regulation of neurofilament length and transport by a dynamic cycle of phospho-dependent polymer severing and annealing.Molecular biology of the cell · 2023Article
- Intermediate filament dysregulation and astrocytopathy in the human disease model ofbioRxiv : the preprint server for biology · 2023Article
- Impact of N-Terminal Tags on De Novo Vimentin Intermediate Filament Assembly.International journal of molecular sciences · 2022Article
- Aberrant histone deacetylase 1 expression upregulates vimentin expression via an NF-κB-dependent pathway in hepatocellular carcinoma.Oncology letters · 2019Article
- Charcot-Marie-Tooth disease Type 2E/1F mutant neurofilament proteins assemble into neurofilaments.Cytoskeleton (Hoboken, N.J.) · 2019Article
- Filaments and phenotypes: cellular roles and orphan effects associated with mutations in cytoplasmic intermediate filament proteins.F1000Research · 2019Review
- Live-cell imaging of neurofilament transport in cultured neurons.Methods in cell biology · 2016Article
- Vimentin filament precursors exchange subunits in an ATP-dependent manner.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Mediated coalescence: a possible mechanism for tumor cellular heterogeneity.American journal of cancer research · 2015Article
- Microtubule-dependent transport of vimentin filament precursors is regulated by actin and by the concerted action of Rho- and p21-activated kinases.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Identification and cytoprotective function of a novel nestin isoform, Nes-S, in dorsal root ganglia neurons.The Journal of biological chemistry · 2013Article
- Withania somnifera root extract inhibits mammary cancer metastasis and epithelial to mesenchymal transition.PloS one · 2013Article
- Article
- Cytoskeletal coherence requires myosin-IIA contractility.Journal of cell science · 2010Article
- A novel recessive Nefl mutation causes a severe, early-onset axonal neuropathy.Annals of neurology · 2009Article
- Intermediate filaments exchange subunits along their length and elongate by end-to-end annealing.The Journal of cell biology · 2009Article
- An aggregate-inducing peripherin isoform generated through intron retention is upregulated in amyotrophic lateral sclerosis and associated with disease pathology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Human cells were transfected with a mouse vimentin cDNA expression vector containing the hormone response element of mouse mammary tumor virus. The distribution of mouse vimentin after induction with dexamethasone was examined by indirect immunofluorescence with antivimentin antibodies specific for either mouse or human vimentin. In stably transfected HeLa cells, which contain vimentin filaments, addition of dexamethasone resulted in the initial appearance of mouse vimentin in discrete areas, usually perinuclear, that always corresponded to areas of the human filament network with the most intense fluorescence. Within 20 h after addition of dexamethasone, the mouse and human vimentin immunofluorescence patterns were identical. However, in stably transfected MCF-7 cells, which lack vimentin filaments, induction of mouse vimentin synthesis resulted in assembly of vimentin filaments throughout the cytoplasm without any obvious local concentrations. Transient expression experiments with SW-13 cell subclones that either lack or contain endogenous vimentin filaments yielded similar results to those obtained with MCF-7 and HeLa transfectants, respectively. Further experiments with HeLa transfectants were conducted to follow the fate of the mouse protein after synthesis had dropped after withdrawal of dexamethasone. The mouse vimentin-specific fluorescence was initially lost from peripheral areas of the cells while the last detectable mouse vimentin always corresponded to the human filament network with the most intense fluorescence. These studies are consistent with a uniform assembly of vimentin filaments throughout the cytoplasm and suggest that previous observations of polarized or vectorial assembly from a perinuclear area to more peripheral areas in cells may be attributable to the nonuniformly distributed appearance of vimentin filaments in immunofluorescence microscopy.
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