ArticleProceedings of the National Academy of Sciences of the United States of America1990
Inhibition of T-cell proliferation by a MYB antisense oligomer is accompanied by selective down-regulation of DNA polymerase alpha expression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 96 citations in OpenAlex.
- Control of plant cytokinesis by an NPK1-mediated mitogen-activated protein kinase cascade.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2002Review
- G2/M-phase-specific transcription during the plant cell cycle is mediated by c-Myb-like transcription factors.The Plant cell · 2001Article
- Constitutive c-Myb amino-terminal phosphorylation and DNA binding activity uncoupled during entry and passage through the cell cycle.Oncogene · 2001Article
- HTLV-I Tax transrepresses the human c-Myb promoter independently of its interaction with CBP or p300.Oncogene · 2000Article
- Review
- Effect of cisplatin and c-myb antisense phosphorothioate oligodeoxynucleotides combination on a human colon carcinoma cell line in vitro and in vivo.British journal of cancer · 1996Article
- The B cell-specific transcription factor BSAP regulates B cell proliferation.The Journal of experimental medicine · 1994Article
- Inhibition of leukaemia cell proliferation by folic acid-polylysine-mediated introduction of c-myb antisense oligodeoxynucleotides into HL-60 cells.British journal of cancer · 1994Article
- DNA and redox state induced conformational changes in the DNA-binding domain of the Myb oncoprotein.The EMBO journal · 1993Article
- Single intraluminal delivery of antisense cdc2 kinase and proliferating-cell nuclear antigen oligonucleotides results in chronic inhibition of neointimal hyperplasia.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Transcription regulation by murine B-myb is distinct from that by c-myb.Nucleic acids research · 1993Article
- Receptor-mediated oligodeoxynucleotides delivery by estradiol and folic acid polylysine conjugates.Cytotechnology · 1993Article
- Regulation of BALB/c 3T3 fibroblast proliferation by B-myb is accompanied by selective activation of cdc2 and cyclin D1 expression.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Growth arrest induced by wild-type p53 protein blocks cells prior to or near the restriction point in late G1 phase.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Cloning of the gene for the 73 kD subunit of the DNA polymerase alpha primase of Drosophila melanogaster.Nucleic acids research · 1992Article
- The role of the promoter in the expression of the PCNA gene.Gene expression · 1992Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We recently found that inhibition of MYB protein synthesis in human peripheral blood mononuclear cells (PBMC) exposed to human c-myb (designated MYB) antisense oligodeoxynucleotides prevents entry into S phase and cell proliferation. To determine the mechanism(s) by which down-regulation of human c-myb protein (MYB) synthesis interferes with DNA synthesis, we analyzed mRNA levels of DNA polymerase alpha and proliferating cell nuclear antigen (PCNA), transcripts of two genes required for DNA synthesis, in normal and leukemic T lymphocytes exposed to MYB antisense oligodeoxynucleotides. Expression of DNA polymerase alpha was inhibited both in normal T lymphocytes progressing from G0 to S phase and in exponentially growing CCRF-CEM leukemic cells, whereas expression of PCNA was inhibited only in mitogen-stimulated PBMC and remained essentially unaffected in the leukemia T-cell line. The functional link between expression of MYB and DNA polymerase alpha mRNAs was further demonstrated by analyzing DNA polymerase alpha mRNA levels in a temperature-sensitive (ts) fibroblast cell line (TK-ts13; TK is thymidine kinase) constitutively expressing human MYB mRNA driven by the simian virus 40 (SV40) promoter. In the MYB-expressing TK-ts13 cells, DNA polymerase alpha mRNA levels were unaffected following shift to the nonpermissive temperature of 39.6 degrees C, whereas in the parental line, DNA polymerase alpha mRNA levels were readily down-regulated. These findings indicate that the expression of MYB is related to that of DNA polymerase alpha in cells expressing MYB at high levels and suggest that there is a functional link between c-myb and DNA polymerase alpha mRNA expression during cell cycle progression of normal T lymphocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.