ArticleRNA (New York, N.Y.)2006
The abundance of the spliceosomal snRNPs is not limiting the splicing of U12-type introns.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Assembly of exon junction complexes on spliceosomes begins before spliceosome activation.Nucleic acids research · 2026Article
- Splicing efficiency of minor introns in a mouse model of SMA predominantly depends on their branchpoint sequence and can involve the contribution of major spliceosome components.RNA (New York, N.Y.) · 2022Article
- IntEREst: intron-exon retention estimator.BMC bioinformatics · 2018Article
- The emerging role of minor intron splicing in neurological disorders.Cell stress · 2018Review
- Alternative exon definition events control the choice between nuclear retention and cytoplasmic export of U11/U12-65K mRNA.PLoS genetics · 2017Article
- Compound heterozygous mutations in the noncoding RNU4ATAC cause Roifman Syndrome by disrupting minor intron splicing.Nature communications · 2015Article
- Global analysis of the nuclear processing of transcripts with unspliced U12-type introns by the exosome.Nucleic acids research · 2014Article
- Defective minor spliceosome mRNA processing results in isolated familial growth hormone deficiency.EMBO molecular medicine · 2014Article
- Article
- Spinal muscular atrophy: new findings for an old pathology.Brain pathology (Zurich, Switzerland) · 2013Review
- Non-polyadenylated transcription in embryonic stem cells reveals novel non-coding RNA related to pluripotency and differentiation.Nucleic acids research · 2013Article
- Tudor staphylococcal nuclease (Tudor-SN) participates in small ribonucleoprotein (snRNP) assembly via interacting with symmetrically dimethylated Sm proteins.The Journal of biological chemistry · 2012Article
- New connections between splicing and human disease.Trends in genetics : TIG · 2012Review
- Coupled pre-mRNA and mRNA dynamics unveil operational strategies underlying transcriptional responses to stimuli.Molecular systems biology · 2011Article
- Requirement of TFIIH kinase subunit Mat1 for RNA Pol II C-terminal domain Ser5 phosphorylation, transcription and mRNA turnover.Nucleic acids research · 2011Article
- Gene expression profiling of U12-type spliceosome mutant Drosophila reveals widespread changes in metabolic pathways.PloS one · 2010Article
- Genome-wide analysis of recombination machinery for spliceosomal introns gain.Molecular biology reports · 2010Article
- Minor spliceosome components are predominantly localized in the nucleus.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The U11-48K protein contacts the 5' splice site of U12-type introns and the U11-59K protein.Molecular and cellular biology · 2008Article
- Transcriptional co-activator protein p100 interacts with snRNP proteins and facilitates the assembly of the spliceosome.Nucleic acids research · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rate of excision of U12-type introns has been reported to be slower than that of U2-type introns, suggesting a rate-limiting bottleneck that could down-regulate genes containing U12-type introns. The mechanistic reasons for this slower rate of intron excision are not known, but lower abundance of the U12-type snRNPs and slower rate of assembly or catalytic activity have been suggested. To investigate snRNP abundance we concentrated on the U4atac snRNA, which is the least abundant of the U12-type snRNAs and is limiting the formation of U4atac/U6atac complex. We identified mouse NIH-3T3 cell line isolates in which the level of both U4atac snRNA and U4atac/U6atac complexes is reduced to 10%-20% of the normal level. We used these cell lines to investigate splicing efficiency by transient transfection of a reporter gene containing a U12-type intron and by quantitative PCR analysis of endogenous genes. The splicing of the reporter U12-type intron was very inefficient, but the activity could be restored by overexpression of U4atac snRNA. Using these U4atac-deficient NIH-3T3 cells, we confirmed the results of previous studies showing that U12-type introns of endogenous genes are, indeed, excised more slowly than U2-type introns, but we found that the rate did not differ from that measured in cells displaying normal levels of U4atac snRNA. Thus our results suggest that the cellular abundance of the snRNPs does not limit U12-type intron splicing under normal conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.