Evidence map›Paper›PMID 16957277›Full record

ArticleApplied and environmental microbiology2006

Molecular-beacon multiplex real-time PCR assay for detection of Vibrio cholerae.

Aneta J Gubala, David F Proll

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Simultaneous Quantification ofPathogens (Basel, Switzerland) · 2020
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Molecular beacons in diagnostics.F1000 medicine reports · 2012
    Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Aneta J GubalaHuman Protection and Performance Division, Defence Science and Technology Organisation, Melbourne, Australia. aneta.gubala@dsto.defence.gov.au
David F Proll

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity and sensitivity of this assay, when tested with pure culture and spiked environmental water samples, were high, surpassing those of currently published PCR assays for the detection of this organism.

Indexed as

Base SequenceDNA, BacterialDNA ProbesGenes, BacterialGenes, RegulatorHumansPolymerase Chain ReactionSensitivity and SpecificityVibrio choleraeVirulenceWater MicrobiologyDNA, BacterialDNA Probes

Identifiers

PMID16957277
PMCPMC1563670

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.