ArticleApplied and environmental microbiology2006
Assessment of genes associated with Streptococcus mutans biofilm morphology.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 62 citations in OpenAlex.
- Morphological changes in Streptococcus mutans after chewing gum containing xylitol for twelve months.Current microbiology · 2009Trial
- Key Salivary Proteins Implicated in the Inhibition of Staphylococcus Aureus Biofilms by Chlorogenic Acid.European journal of dentistry · 2026Article
- Prevalence of oral streptococci and glucosyltransferase genes in mother-child pairs: a cross-sectional study in Turkish families.BMC pediatrics · 2026Article
- The Role and Progress of Antimicrobial Peptides in Managing Oral Biofilms: A Narrative Review.International dental journal · 2026Review
- Formation of Mono-Organismal and MixedMicroorganisms · 2025Article
- Marine-derived κ-carrageenan-coated zinc oxide nanoparticles for targeted drug delivery and apoptosis induction in oral cancer.Molecular biology reports · 2024Article
- Investigation of Components in Roasted Green Tea That InhibitFoods (Basel, Switzerland) · 2023Article
- Inhibitory effect of the combination of xylitol and funoran on Streptococcus mutans biofilm formation on the uncoated surface.Archives of microbiology · 2022Article
- Effects of pH on the Properties of Membrane Vesicles Including Glucosyltransferase inMicroorganisms · 2021Article
- Isolation and Characterization ofViruses · 2021Article
- Lantibiotics Produced by Oral Inhabitants as a Trigger for Dysbiosis of Human Intestinal Microbiota.International journal of molecular sciences · 2021Article
- Effects of Complex DNA and MVs with GTF Extracted fromMolecules (Basel, Switzerland) · 2019Article
- The inhibitory effects of polypyrrole on the biofilm formation of Streptococcus mutans.PloS one · 2019Article
- Role of SCFAs for Fimbrillin-Dependent Biofilm Formation ofMicroorganisms · 2018Article
- Raffinose Induces Biofilm Formation by Streptococcus mutans in Low Concentrations of Sucrose by Increasing Production of Extracellular DNA and Fructan.Applied and environmental microbiology · 2017Article
- Effect of the Biofilm Age and Starvation on Acid Tolerance of Biofilm Formed by Streptococcus mutans Isolated from Caries-Active and Caries-Free Adults.International journal of molecular sciences · 2017Article
- Comparative analysis of prophages inPeerJ · 2017Article
- Article
- Biological cost of different mechanisms of colistin resistance and their impact on virulence in Acinetobacter baumannii.Antimicrobial agents and chemotherapy · 2014Article
- Competence-dependent endogenous DNA rearrangement and uptake of extracellular DNA give a natural variant of Streptococcus mutans without biofilm formation.Journal of bacteriology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus mutans, the major pathogen responsible for dental caries in humans, is a biofilm-forming bacterium. In the present study, 17 different pulsed-field gel electrophoresis patterns of genomic DNA were identified in S. mutans organisms isolated clinically from whole saliva. The S. mutans isolates showed different abilities to form biofilms on polystyrene surfaces in semidefined minimal medium cultures. Following cultivation in a flow cell system in tryptic soy broth with 0.25% sucrose and staining using a BacLight LIVE/DEAD system, two strains, designated FSC-3 and FSC-4, showed the greatest and least, respectively, levels of biofilm formation when examined with confocal laser scanning microscopy. Further, image analyses of spatial distribution and architecture were performed to quantify the merged green (live cells) and red (dead cells) light. The light intensity of the FSC-3 biofilm was greater than that of the FSC-4 biofilm in the bottom area but not in the top area. S. mutans whole-genome array results showed that approximately 3.8% of the genes were differentially expressed in the two strains, of which approximately 2.2%, including bacitracin transport ATP-binding protein gene glrA and a BLpL-like putative immunity protein gene, were activated in FSC-3. In addition, about 1.6% of the genes, including those associated with phosphotransferase system genes, were repressed. Analyses of the glrA-deficient strains and reverse transcription-PCR confirmed the role of the gene in biofilm formation. Differential assessment of biofilm-associated genes in clinical strains may provide useful information for understanding the morphological development of streptococcal biofilm, as well as for colonization of S. mutans.
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