ArticleApplied and environmental microbiology2006
Reductive dehalogenase gene expression as a biomarker for physiological activity of Dehalococcoides spp.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 116 citations in OpenAlex.
- Developing a microbial community structure index (MCSI) as an approach to evaluate and optimize bioremediation performance.Biodegradation · 2024Article
- Metabolome patterns identify active dechlorination in bioaugmentation consortium SDC-9™.Frontiers in microbiology · 2022Article
- Biological effects of tourmaline treatment onRSC advances · 2021Article
- Iron Sulfide Enhanced the Dechlorination of Trichloroethene byFrontiers in microbiology · 2021Article
- Structural dynamics and transcriptomic analysis of Dehalococcoides mccartyi within a TCE-Dechlorinating community in a completely mixed flow reactor.Water research · 2019Article
- Microbial Community Changes in a Chlorinated Solvents Polluted Aquifer Over the Field Scale Treatment With Poly-3-Hydroxybutyrate as Amendment.Frontiers in microbiology · 2018Article
- Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities.Applied and environmental microbiology · 2017Article
- Acetylene Fuels TCE Reductive Dechlorination by Defined Dehalococcoides/Pelobacter Consortia.Environmental science & technology · 2017Article
- PCBs attenuation and abundance of Dehalococcoides spp., bphC, CheA, and flic genes in typical polychlorinated biphenyl-polluted soil under floody and dry soil conditions.Environmental science and pollution research international · 2016Article
- Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation.Frontiers in microbiology · 2016Review
- Identification of specific corrinoids reveals corrinoid modification in dechlorinating microbial communities.Environmental microbiology · 2015Article
- A comparative genomics and reductive dehalogenase gene transcription study of two chloroethene-respiring bacteria, Dehalococcoides mccartyi strains MB and 11a.Scientific reports · 2015Article
- Efficient metabolic exchange and electron transfer within a syntrophic trichloroethene-degrading coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei.Applied and environmental microbiology · 2015Article
- Chloroethene degradation and expression of Dehalococcoides dehalogenase genes in cultures originating from Yangtze sediments.Environmental science and pollution research international · 2015Article
- Identification of biomarker genes to predict biodegradation of 1,4-dioxane.Applied and environmental microbiology · 2014Article
- Article
- Assessment of anaerobic toluene biodegradation activity by bssA transcript/gene ratios.Applied and environmental microbiology · 2013Article
- Characterization of four TCE-dechlorinating microbial enrichments grown with different cobalamin stress and methanogenic conditions.Applied microbiology and biotechnology · 2013Article
- Organic cofactors in the metabolism of Dehalococcoides mccartyi strains.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- Metagenomic analysis of a stable trichloroethene-degrading microbial community.The ISME journal · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
This study characterizes the transcriptional expression of the reductive dehalogenase (RDase)-encoding tceA and vcrA genes and evaluates their applicability as potential biological markers of Dehalococcoides activity. When Dehalococcoides ethenogenes 195 was provided with trichloroethene (TCE) as the electron acceptor, the expression of the tceA gene increased by 90-fold relative to that in cells starved of chlorinated ethenes, demonstrating that tceA gene expression is indicative of the active physiological state of this strain. In a Dehalococcoides-containing enrichment culture that contains both the tceA and vcrA genes, the tceA gene was up-regulated in response to TCE and cis-1,2-dichloroethene (cDCE) exposure, while the vcrA gene was up-regulated in response to TCE, cDCE, and vinyl chloride (VC). When chlorinated ethenes were depleted, the RDase-encoding gene transcripts decayed exponentially, with a half-life between 4.8 and 6.1 h, until they reached a stable background level after 2 days. We found that while gene expression correlated generally to the presence of chlorinated ethenes, there was no apparent direct relationship between RDase-encoding transcript numbers and respective rates of TCE, cDCE, and VC dechlorination activities. However, elevated tceA and vcrA expression did correlate with chlorinated-ethene reduction beyond cDCE, suggesting that elevated RDase-encoding transcript numbers could serve as a biomarker for the physiological ability of Dehalococcoides spp. to dechlorinate beyond cDCE.
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