ArticleApplied and environmental microbiology2006
Rapid quantitative detection of Lactobacillus sakei in meat and fermented sausages by real-time PCR.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 63 citations in OpenAlex.
- A Novel qPCR Method for the Detection of Lactic Acid Bacteria in Fermented Milk.Foods (Basel, Switzerland) · 2021Article
- Growth and Expression of Virulence Genes ofBiology · 2021Article
- Multiplex Real-Time PCR with HRM for Detection ofFood technology and biotechnology · 2019Article
- Development of a rapid real-time PCR method as a tool to quantify viable Photobacterium phosphoreum bacteria in salmon (Salmo salar) steaks.Applied and environmental microbiology · 2013Article
- Article
- Quantification of Propionibacterium acidipropionici P169 bacteria in environmental samples by use of strain-specific primers derived by suppressive subtractive hybridization.Applied and environmental microbiology · 2011Article
- Microbial deterioration of vacuum-packaged chilled beef cuts and techniques for microbiota detection and characterization: a review.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- Application of quantitative real-time PCR for enumeration of total bacterial, archaeal, and yeast populations in kimchi.Journal of microbiology (Seoul, Korea) · 2009Article
- Culture-independent identification of gut bacteria correlated with the onset of diabetes in a rat model.The ISME journal · 2009Article
- A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards.BMC bioinformatics · 2008Article
- Development of quantitative real-time PCR assays for detection and quantification of surrogate biological warfare agents in building debris and leachate.Applied and environmental microbiology · 2007Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A quick and simple method for quantitative detection of Lactobacillus sakei in fermented sausages was successfully developed. It is based on Chelex-100-based DNA purification and real-time PCR enumeration using a TaqMan fluorescence probe. Primers and probes were designed in the L. sakei 16S-23S rRNA intergenic transcribed spacer region, and the assay was evaluated using L. sakei genomic DNA and an artificially inoculated sausage model. The detection limit of this technique was approximately 3 cells per reaction mixture using both purified DNA and the inoculated sausage model. The quantification limit was established at 30 cells per reaction mixture in both models. The assay was then applied to enumerate L. sakei in real samples, and the results were compared to the MRS agar count method followed by confirmation of the percentage of L. sakei colonies. The results obtained by real-time PCR were not statistically significantly different than those obtained by plate count on MRS agar (P > 0.05), showing a satisfactory agreement between both methods. Therefore, the real-time PCR assay developed can be considered a promising rapid alternative method for the quantification of L. sakei and evaluation of the implantation of starter strains of L. sakei in fermented sausages.
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