ArticleApplied and environmental microbiology2006
Detection of Cryptosporidium oocysts in water: effect of the number of samples and analytic replicates on test results.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Article
- Detection ofMicroorganisms · 2022Review
- Universal Enzyme-Based Field Workflow for Rapid and Sensitive Quantification of Water Pathogens.Microorganisms · 2021Article
- Occurrence of Intestinal Parasites of Public Health Significance in Fresh Horticultural Products Sold in Maputo Markets and Supermarkets, Mozambique.Microorganisms · 2021Article
- Establishment and preliminary application of nanoparticle-assisted PCR assay for detection of Cryptosporidium spp.Parasitology research · 2021Article
- Label-Free Capacitive Biosensor for Detection ofSensors (Basel, Switzerland) · 2019Article
- Subtype analysis of zoonotic pathogen Cryptosporidium skunk genotype.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2017Article
- Fine-Scale Spatial Heterogeneity in the Distribution of Waterborne Protozoa in a Drinking Water Reservoir.International journal of environmental research and public health · 2015Article
- Comparison of nested PCR and microscopy for the detection of cryptosporidiosis in bovine calves.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2014Article
- Effective concentration and detection of cryptosporidium, giardia, and the microsporidia from environmental matrices.Journal of pathogens · 2014Article
- Genetic diversity of Cryptosporidium spp. within a remote population of Soay Sheep on St. Kilda Islands, Scotland.Applied and environmental microbiology · 2013Article
- Occurrences and genotypes of Cryptosporidium oocysts in river network of southern-eastern China.Parasitology research · 2012Article
- Genotypic characterization of Cryptosporidium hominis from water samples in Sao Paulo, Brazil.The American journal of tropical medicine and hygiene · 2011Article
- Detection and resolution of Cryptosporidium species and species mixtures by genus-specific nested PCR-restriction fragment length polymorphism analysis, direct sequencing, and cloning.Applied and environmental microbiology · 2011Article
- MicroRNA-98 and let-7 regulate expression of suppressor of cytokine signaling 4 in biliary epithelial cells in response to Cryptosporidium parvum infection.The Journal of infectious diseases · 2010Article
- Relationships among bather density, levels of human waterborne pathogens, and fecal coliform counts in marine recreational beach water.Parasitology research · 2010Article
- Propagation of human enteropathogens in constructed horizontal wetlands used for tertiary wastewater treatment.Applied and environmental microbiology · 2009Article
- Cryptosporidium source tracking in the Potomac River watershed.Applied and environmental microbiology · 2008Article
- Molecular characterization of the Cryptosporidium cervine genotype from a sika deer (Cervus nippon Temminck) in Zhengzhou, China and literature review.Parasitology research · 2008Review
- Cryptosporidium genotypes in wildlife from a new york watershed.Applied and environmental microbiology · 2007Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to the small number of Cryptosporidium oocysts in water, the number of samples taken and the analyses performed can affect the results of detection. In this study, 42 water samples were collected from one watershed during 20 storm events over 1 year, including duplicate or quadruplicate samples from 16 storm events. Ten samples from four events had three to eight subsamples. They were processed by EPA method 1623, and Cryptosporidium oocysts present were detected by immunofluorescent microscopy or PCR. Altogether, 24 of 39 samples (47 of 67 samples and subsamples) analyzed by microscopy were positive for Cryptosporidium. In contrast, 36 of 42 samples (62 of 76 samples and subsamples) were positive by PCR, including 10 microscopy-negative samples (13 microscopy-negative samples and subsamples). Six of the 24 microscopy-positive samples were negative by PCR, and all samples had one or less oocyst in a 0.5-ml packed pellet volume calculated. Discordant results were obtained by microscopy and PCR from six and three of the storm events, respectively, with multiple samples. Discordant microscopy or PCR results were also obtained among subsamples. Most of the 14 Cryptosporidium genotypes were found over a brief period. Cryptosporidium-positive samples had a mean of 1.9 genotypes per sample, with 39 of the 62 positive samples/subsamples having more than one genotype. Samples/subsamples with more than one genotype had an overall PCR-positive rate of 73%, compared to 34% for those with one genotype. The PCR amplification rate of samples was affected by the volume of DNA used in PCR.
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